Selective Detection of Alkaline Phosphatase Activity in Environmental Water Samples by Copper Nanoclusters Doped Lanthanide Coordination Polymer Nanocomposites as the Ratiometric Fluorescent Probe

Biosensors

卷:12

Xiaoling Wang¹  Chunyuan Tian¹  Lijun Zhao¹  Xuming Zhuang¹  Feiyan Tang¹  Qing Hua¹  Feng Luan¹  Yunfei Wang¹  Xin Li¹  Wei Guo² 

[1] College of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, China;

[2] Shandong Dyne Marine Biopharmaceutical Co., Ltd., Weihai 264300, China;

关键词: alkaline phosphatase;    copper nanoclusters;    infinite coordination polymer;    ratiometric;    fluorescent probe;   

DOI  :  10.3390/bios12060372

来源: DOAJ

【 摘 要 】

In this paper, a novel, accurate, sensitive and rapid ratiometric fluorescent sensor was fabricated using a copper nanoclusters@infinite coordination polymer (ICP), specifically, terbium ion-guanosine 5’-disodium (Cu NCs@Tb-GMP) nanocomposites as the ratiometric fluorescent probe, to detect alkaline phosphatase (ALP) in water. The fluorescence probe was characterized by scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy. The experimental results showed that, compared with Tb-GMP fluorescent sensors, Cu ratiometric fluorescent sensors based on NCs encapsulated in Tb-GMP had fewer experimental errors and fewer false-positive signals and were more conducive to the sensitive and accurate detection of ALP. In addition, the developed fluorescent probe had good fluorescence intensity, selectivity, repeatability and stability. Under optimized conditions, the ratiometric fluorescent sensor detected ALP in the range of 0.002–2 U mL⁻¹ (R² = 0.9950) with a limit of detection of 0.002 U mL⁻¹, and the recovery of ALP from water samples was less than 108.2%. These satisfying results proved that the ratiometric fluorescent probe has good application prospects and provides a new method for the detection of ALP in real water samples.

【 授权许可】

Unknown