Study of the Lipolysis Effect of Nanoliposome-Encapsulated Ganoderma lucidum Protein Hydrolysates on Adipocyte Cells Using Proteomics Approach

Foods

Phakorn Papan¹  Pawitrabhorn Samutrtai²  Sucheewin Krobthong³  Kiattawee Choowongkomon³  Wonnop Visessanguan⁴  Thanisorn Mahatnirunkul⁵  Yodying Yingchutrakul⁶  Chartchai Chaichana⁷ 

[1] Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand;Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand;Interdisciplinary Graduate Program in Genetic Engineering, Kasetsart University, Bangkok 10900, Thailand;National Center for Genetic Engineering and Biotechnology, NSTDA, Pathum Thani 12120, Thailand;National Nanotechnology Center, NSTDA, Pathum Thani 12120, Thailand;National Omics Center, NSTDA, Pathum Thani 12120, Thailand;Siriraj Center of Research Excellence for Diabetes and Obesity (SiCORE-DO), Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand;

关键词: RSM;    Lingzhi;    hypolipidemic activity;    peptides;    3T3-L1;   

DOI  :  10.3390/foods10092157

来源: DOAJ

【 摘 要 】

Excessive lipid accumulation is a serious condition. Therefore, we aimed at developing safe strategies using natural hypolipidemic products. Lingzhi is an edible fungus and potential lipid suppression stimulant. To use Lingzhi as a functional hyperlipidemic ingredient, response surface methodology (RSM) was conducted to optimize the time (X₁) and enzyme usage (X₂) for the hydrolysate preparation with the highest degree of hydrolysis (DH) and % yield. We encapsulated the hydrolysates using nanoscale liposomes and used proteomics to study how these nano-liposomal hydrolysates could affect lipid accumulation in adipocyte cells. RSM analysis revealed X₁ at 8.63 h and X₂ at 0.93% provided the highest values of DH and % yields were 33.99% and 5.70%. The hydrolysates were loaded into liposome particles that were monodispersed. The loaded nano-liposomal particles did not significantly affect cell survival rates. The triglyceride (TG) breakdown in adipocytes showed a higher TG increase compared to the control. Lipid staining level upon the liposome treatment was lower than that of the control. Proteomics revealed 3425 proteins affected by the liposome treatment, the main proteins being TSSK5, SMU1, GRM7, and KLC4, associated with various biological functions besides lipolysis. The nano-liposomal Linzghi hydrolysate might serve as novel functional ingredients in the treatment and prevention of obesity

【 授权许可】

Unknown