Maternal Dietary Docosahexaenoic Acid Alters Lipid Peroxidation Products and (n-3)/(n-6) Fatty Acid Balance in Offspring Mice

Metabolites

Runting Li¹  GraceY. Sun¹  JamesC. Lee²  Bo Yang³  C.Michael Greenlief³  JimmyD. Browning⁴  KevinL. Fritsche⁴  Hailong Song⁵  Zezong Gu⁵  Jiankun Cui⁵  DavidQ. Beversdorf⁶  Taeseon Woo⁶ 

[1] Biochemistry Department, University of Missouri, Columbia, MO 65211, USA;Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607, USA;Department of Chemistry, University of Missouri, Columbia, MO 65211, USA;Department of Nutrition and Exercise Physiology, University of Missouri, Columbia, MO 65211, USA;Department of Pathology and Anatomical Sciences, University of Missouri, Columbia, MO 65211, USA;Departments of Radiology, Neurology and Psychological Sciences, and the Thompson Center, University of Missouri, Columbia, MO 65211, USA;

关键词: diet and dietary lipids;    arachidonic acid;    omega-3 fatty acids;    lipid peroxidation;    4-hydroxyhexenal;    4-hydroxynonenal;    autism spectrum disorder;    liquid chromatography;    tandem mass spectrometry;   

DOI  :  10.3390/metabo9030040

来源: DOAJ

【 摘 要 】

The abundance of docosahexaenoic acid (DHA) in the mammalian brain has generated substantial interest in the search for its roles in regulating brain functions. Our recent study with a gene/stress mouse model provided evidence to support the ability for the maternal supplement of DHA to alleviate autism-associated behavior in the offspring. DHA and arachidonic acid (ARA) are substrates of enzymatic and non-enzymatic reactions, and lipid peroxidation results in the production of 4-hydroxyhexenal (4-HHE) and 4-hydroxynonenal (4-HNE), respectively. In this study, we examine whether a maternal DHA-supplemented diet alters fatty acids (FAs), as well as lipid peroxidation products in the pup brain, heart and plasma by a targeted metabolite approach. Pups in the maternal DHA-supplemented diet group showed an increase in DHA and a concomitant decrease in ARA in all brain regions examined. However, significant increases in 4-HHE, and not 4-HNE, were found mainly in the cerebral cortex and hippocampus. Analysis of heart and plasma showed large increases in DHA and 4-HHE, but a significant decrease in 4-HNE levels only in plasma. Taken together, the DHA-supplemented maternal diet alters the (n-3)/(n-6) FA ratio, and increases 4-HHE levels in pup brain, heart and plasma. These effects may contribute to the beneficial effects of DHA on neurodevelopment, as well as functional changes in other body organs.

【 授权许可】

Unknown