Bioavailability and spatial distribution of fatty acids in the rat retina after dietary omega-3 supplementation

Journal of Lipid Research

Bokkyoo Jun¹  Olivier Berdeaux²  Stephanie Cabaret²  Niyazi Acar²  Nicolas G. Bazan³  Lucy Martine³  Spiro Khoury³  Lionel Bretillon³  Elisa Vidal³  Stéphane Grégoire³  Marie-Annick Maire⁴  William C. Gordon⁴ 

[1] Horus Pharma Laboratories, Saint Laurent du Var, France;Chemosens Group, Centre des Sciences du Goût et de l'Alimentation, AgroSup Dijon, CNRS, INRAE, Université Bourgogne Franche-Comté, Dijon, France;Eye and Nutrition Research Group, Centre des Sciences du Goût et de l'Alimentation, AgroSup Dijon, INRAE, CNRS, Université Bourgogne Franche-Comté, Dijon, France;Neuroscience Center of Excellence, School of Medicine, Louisiana State University Health New Orleans, New Orleans, LA, USA;

关键词: diet and dietary lipids;    docosahexaenoic acid;    eicosapentaenoic acid;    lipid biochemistry;    lipid spatial organization;    omega-3 fatty acids;   

DOI  :  

来源: DOAJ

【 摘 要 】

Spatial changes of FAs in the retina in response to different dietary n-3 formulations have never been explored, although a diet rich in EPA and DHA is recommended to protect the retina against the effects of aging. In this study, Wistar rats were fed for 8 weeks with balanced diet including either EPA-containing phospholipids (PLs), EPA-containing TGs, DHA-containing PLs, or DHA-containing TGs. Qualitative changes in FA composition of plasma, erythrocytes, and retina were evaluated by gas chromatography-flame ionization detector. Following the different dietary intakes, changes to the quantity and spatial organization of PC and PE species in retina were determined by LC coupled to MS/MS and MALDI coupled to MS imaging. The omega-3 content in the lipids of plasma and erythrocytes suggests that PLs as well as TGs are good omega-3 carriers for retina. However, a significant increase in DHA content in retina was observed, especially molecular species as di-DHA-containing PC and PE, as well as an increase in very long chain PUFAs (more than 28 carbons) following PL-EPA and TG-DHA diets only. All supplemented diets triggered spatial organization changes of DHA in the photoreceptor layer around the optic nerve. Taken together, these findings suggest that dietary omega-3 supplementation can modify the content of FAs in the rat retina.

【 授权许可】

Unknown