Identification and Single‐Cell Analysis of Viable Circulating Tumor Cells by a Mitochondrion‐Specific AIE Bioprobe

Advanced Science

Liyao Mai¹  Siqi Wang¹  Xinghua Pan¹  Qianwen Zhao²  Jing Chen²  Bo Situ²  Ye Zhang²  Lei Zheng²  Xinyi Ye²  Bo Li²  Bairong He²  Yifang Huang²  Jianjun Zou³  Ben Zhong Tang⁴ 

[1] Department of Biochemistry and Molecular Biology School of Basic Medical Sciences Southern Medical University Guangzhou 510515 China;Department of Laboratory Medicine Nanfang Hospital Southern Medical University Guangzhou 510515 China;Department of Oncology Guangzhou Chest Hospital Guangzhou 510515 China;Guangdong Province Key Laboratory of Biomedical Engineering South China University of Technology Guangzhou 510006 China;

关键词: aggregation‐induced emission;    circulating tumor cells;    fluorescent probes;    single‐cell analysis;   

DOI  :  10.1002/advs.201902760

来源: DOAJ

【 摘 要 】

Abstract Liquid biopsies of cancer via single‐cell molecular profiling of circulating tumor cells (CTCs) are hampered by the lack of ideal CTC markers. In this study, it is reported that TPN, a bioprobe with aggregation‐induced emission (AIE) activity is capable of distinguishing various tumor cells from blood leukocytes based on the difference in cell mitochondria. TPN is a cell‐permeant live‐cell stain that has little effect on cell viability and integrity, enabling single‐cell DNA/RNA analysis with improved efficiency compared with traditional antibody‐based methods. Using TPN labeling, CTCs and CTC cluster are detected in the blood from patients with lung or liver cancer. The capability of TPN to identify rare tumor cells in the malignant pleural effusion samples is also demonstrated. Furthermore, RNA sequencing of single lung CTC identified by TPN is successfully performed. The findings presented here provide an antibody‐free, low‐cost, and nondisruptive approach for detection and genomic characterization of viable tumor cells based on a mitochondria‐targeting AIE luminogen. It might serve as a new tool for monitoring of genomics dynamic of tumor and unraveling the mechanisms of tumor metastasis.

【 授权许可】

Unknown