Piriformospora indica Primes Onion Response against Stemphylium Leaf Blight Disease

Pathogens

Massimiliano Morelli¹  Kiran Khandagale²  Avinash Ade²  Suresh Gawande³  Praveen Roylawar³  Major Singh³  Pragati Randive³  Chandrashekhar Murumkar⁴  Bharat Shinde⁵ 

[1] CNR-IPSP Istituto per la Protezione Sostenibile delle Piante, Sede Secondaria di Bari, 70124 Bari, Italy;Department of Botany, Savitribai Phule Pune University, Pune 411007, India;ICAR-Directorate of Onion and Garlic Research (DOGR), Rajgurunagar, Pune 410505, India;Tuljaram Chaturchand College of Arts, Science and Commerce, Baramati, Pune 413102, India;Vidya Pratishthan’s Arts, Science & Commerce College, Baramati, Pune 413133, India;

关键词: biocontrol;    Induced systemic resistance;    onion;    Piriformospora indica;    Stemphylium leaf blight disease;    Stemphylium vesicarium;   

DOI  :  10.3390/pathogens10091085

来源: DOAJ

【 摘 要 】

The root-endophytic fungus Piriformospora indica (=Serendipita indica) has been revealed for its growth-promoting effects and its capacity to induce resistance in a broad spectrum of host plants. However, the bioefficacy of this fungus had not yet been tested against any pathogen affecting onion (Allium cepa). In this study, the biocontrol potency of P. indica against onion leaf blight, an impacting disease caused by the necrotrophic fungal pathogen Stemphylium vesicarium, was evaluated. First, it was proved that colonisation of onion roots by P. indica was beneficial for plant growth, as it increased leaf development and root biomass. Most relevantly, P. indica was also effective in reducing Stemphylium leaf blight (SLB) severity, as assessed under greenhouse conditions and confirmed in field trials in two consecutive years. These investigations could also provide some insight into the biochemical and molecular changes that treatment with P. indica induces in the main pathways associated with host defence response. It was possible to highlight the protective effect of P. indica colonisation against peroxidative damage, and its role in signalling oxidative stress, by assessing changes in malondialdehyde and H₂O₂ content. It was also showed that treatment with P. indica contributes to modulate the enzymatic activity of superoxide dismutase, catalase, phenylalanine ammonia-lyase and peroxidase, in the course of infection. qPCR-based expression analysis of defence-related genes AcLOX1, AcLOX2, AcPAL1, AcGST, AcCHI, AcWRKY1, and AcWRKY70 provided further indications on P. indica ability to induce onion systemic response. Based on the evidence gathered, this study aims to propose P. indica application as a sustainable tool for improving SLB control, which might not only enhance onion growth performance but also activate defence signalling mechanisms more effectively, involving different pathways.

【 授权许可】

Unknown