The gut bacterial microbiome of Nile tilapia (Oreochromis niloticus) from lakes across an altitudinal gradient

BMC Microbiology

Getachew Beneberu Abebe¹  Manuel Curto²  Harald Meimberg²  Herwig Waidbacher³  Solomon Workneh Fanta⁴  Negash Kabtimer Bereded⁵  Konrad J. Domig⁵ 

[1] Department of Biology, Bahir Dar University;Department of Integrative Biology and Biodiversity Research, Institute for Integrative Nature Conservation Research;Department of Water, Atmosphere and Environment, Institute of Hydrobiology and Aquatic Ecosystem Management;Faculty of Chemical and Food Engineering, Bahir Dar Institute of Technology, Bahir Dar University;University of Natural Resources and Life Sciences;

关键词: Altitude;    Diversity;    Fish;    Gut microbiota;    Lake;    16S rDNA;   

DOI  :  10.1186/s12866-022-02496-z

来源: DOAJ

【 摘 要 】

Abstract Background Microorganisms inhabiting the gut play a significant role in supporting fundamental physiological processes of the host, which contributes to their survival in varied environments. Several studies have shown that altitude affects the composition and diversity of intestinal microbial communities in terrestrial animals. However, little is known about the impact of altitude on the gut microbiota of aquatic animals. The current study examined the variations in the gut microbiota of Nile tilapia (Oreochromis niloticus) from four lakes along an altitudinal gradient in Ethiopia by using 16S rDNA Illumina MiSeq high-throughput sequencing. Results The results indicated that low-altitude samples typically displayed greater alpha diversity. The results of principal coordinate analysis (PCoA) showed significant differences across samples from different lakes. Firmicutes was the most abundant phylum in the Lake Awassa and Lake Chamo samples whereas Fusobacteriota was the dominant phylum in samples from Lake Hashengie and Lake Tana. The ratio of Firmicutes to Bacteroidota in the high-altitude sample (Lake Hashengie, altitude 2440 m) was much higher than the ratio of Firmicutes to Bacteroidota in the low altitude population (Lake Chamo, altitude 1235 m). We found that the relative abundances of Actinobacteriota, Chloroflexi, Cyanobacteria, and Firmicutes were negatively correlated with altitude, while Fusobacteriota showed a positive association with altitude. Despite variability in the abundance of the gut microbiota across the lakes, some shared bacterial communities were detected. Conclusions In summary, this study showed the indirect influence of altitude on gut microbiota. Altitude has the potential to modulate the gut microbiota composition and diversity of Nile tilapia. Future work will be needed to elucidate the functional significance of gut microbiota variations based on the geographical environment. Significance and impact of the study Our study determined the composition and diversity of the gut microbiota in Nile tilapia collected from lakes across an altitude gradient. Our findings greatly extend the baseline knowledge of fish gut microbiota in Ethiopian lakes that plays an important role in this species sustainable aquaculture activities and conservation.

【 授权许可】

Unknown