Relative abundance of the Prevotella genus within the human gut microbiota of elderly volunteers determines the inter-individual responses to dietary supplementation with wheat bran arabinoxylan-oligosaccharides

BMC Microbiology

Douwina Bosscher¹  Vicenta Garcia-Campayo²  Julian Parkhill³  Alan W. Walker⁴  Harry J. Flint⁴  Wing Sun Faith Chung⁴  Sylvia H. Duncan⁴  Josef Wagner⁵ 

[1]Cargill R&D Centre Europe BVBA, Havenstraat 84, B-1800, Vilvoorde, Belgium

[2]Cargill, Incorporated, PO Box 9300, 55440-9300, Minneapolis, MN, USA

[3]Department of Veterinary Medicine, University of Cambridge, Madingley Road, CB3 0ES, Cambridge, UK

[4]Gut Health Group, Rowett Institute, University of Aberdeen, Foresterhill, AB25 2ZD, Aberdeen, Scotland, UK

[5]Pathogen Genomics Group, Wellcome Sanger Institute, Hinxton, CB10 1SA, Cambridgeshire, UK

关键词: Gut microbiota;    Diversity;    Bacteroides;    Prevotella;    Bifidobacteria;    Wheatbran extract;    Arabinoxylan oligosaccharides (AXOS);    Short chain fatty acids;    Propionate;    Butyrate;   

DOI  :  10.1186/s12866-020-01968-4

来源: Springer

【 摘 要 】

BackgroundThe human colon is colonised by a dense microbial community whose species composition and metabolism are linked to health and disease. The main energy sources for colonic bacteria are dietary polysaccharides and oligosaccharides. These play a major role in modulating gut microbial composition and metabolism, which in turn can impact on health outcomes.ResultsWe investigated the influence of wheat bran arabinoxylan oligosaccharides (AXOS) and maltodextrin supplements in modulating the composition of the colonic microbiota and metabolites in healthy adults over the age of 60. Male and female volunteers, (n = 21, mean BMI 25.2 ± 0.7 kg/m2) participated in the double-blind, cross over supplement study. Faecal samples were collected for analysis of microbiota, short chain fatty acids levels and calprotectin. Blood samples were collected to measure glucose, cholesterol and triglycerides levels. There was no change in these markers nor in calprotectin levels in response to the supplements. Both supplements were well-tolerated by the volunteers. Microbiota analysis across the whole volunteer cohort revealed a significant increase in the proportional abundance of faecal Bifidobacterium species (P ≤ 0.01) in response to AXOS, but not maltodextrin, supplementation. There was considerable inter-individual variation in the other bacterial taxa that responded, with a clear stratification of volunteers as either Prevotella-plus (n = 8; > 0.1% proportional abundance) or Prevotella-minus (n = 13; ≤0.1% proportional abundance) subjects founded on baseline sample profiles. There was a significant increase in the proportional abundance of both faecal Bifidobacterium (P ≤ 0.01) and Prevotella species (P ≤ 0.01) in Prevotella-plus volunteers during AXOS supplementation, while Prevotella and Bacteroides relative abundances showed an inverse relationship. Proportional abundance of 26 OTUs, including bifidobacteria and Anaerostipes hadrus, differed significantly between baseline samples of Prevotella-plus compared to Prevotella-minus individuals.ConclusionsThe wheat bran AXOS supplementation was bifidogenic and resulted in changes in human gut microbiota composition that depended on the initial microbiota profile, specifically the presence or absence of Prevotella spp. as a major component of the microbiota. Our data therefore suggest that initial profiling of individuals through gut microbiota analysis should be considered important when contemplating nutritional interventions that rely on prebiotics.Trial registrationClinical trial registration number: NCT02693782. Registered 29 February 2016 - Retrospectively registered, https://clinicaltrials.gov/ct2/show/NCT02693782?term=NCT02693782&rank=1

【 授权许可】

CC BY