LC-MS-based method for the qualitative and quantitative analysis of complex lipid mixtures

Journal of Lipid Research

Francine K. Welty¹  Haya Herscovitz²  Ulf Sommer³  Catherine E. Costello³ 

[1] Cardiovascular Division, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA;Department of Physiology and Biophysics, Boston University School of Medicine, Boston, MA;Mass Spectrometry Resource, Department of Biochemistry, Boston University School of Medicine, Boston, MA;

关键词: low density lipoprotein;    intermediate density lipoprotein;    normal-phase high-performance liquid chromatography-mass spectrometry;    reversed-phase liquid chromatography-tandem mass spectrometry;    familial hypobetalipoproteinemia;   

DOI  :  

来源: DOAJ

【 摘 要 】

A simple and robust LC-MS-based methodology for the investigation of lipid mixtures is described, and its application to the analysis of human lipoprotein-associated lipids is demonstrated. After an optional initial fractionation on Silica 60, normal-phase HPLC-MS on a YMC PVA-Sil column is used first for class separation, followed by reversed-phase LC-MS or LC-tandem mass spectrometry using an Atlantis dC18 capillary column, and/or nanospray MS, to fully characterize the individual lipids. The methodology is applied here for the analysis of human apolipoprotein B-associated lipids. This approach allows for the determination of even low percentages of lipids of each molecular species and showed clear differences between lipids associated with apolipoprotein B-100-LDL isolated from a normal individual and those associated with a truncated version, apolipoprotein B-67-containing lipoproteins, isolated from a homozygote patient with familial hypobetalipoproteinemia. The methods described should be easily adaptable to most modern MS instrumentation.

【 授权许可】

Unknown