The Enzymatic and Non-Enzymatic Antioxidant System Response of the Seagrass Cymodocea nodosa to Bisphenol-A Toxicity

International Journal of Molecular Sciences

Paraskevi Malea¹  Danae Kokkinidi¹  Alkistis Kevrekidou²  Ioannis-Dimosthenis S. Adamakis³ 

[1] School of Biology, Department of Botany, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece;School of Engineering, Department of Chemical Engineering, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece;Section of Botany, Department of Biology, National and Kapodistrian University of Athens, 15784 Athens, Greece;

关键词: ascorbate peroxidase activity;    BPA;    hydrogen peroxide;    lipid peroxidation;    phenolic compounds;    superoxide dismutase activity;   

DOI  :  10.3390/ijms23031348

来源: DOAJ

【 摘 要 】

The effects of environmentally relevant bisphenol A (BPA) concentrations (0.3, 1 and 3 μg L⁻¹) were tested at 2, 4, 6 and 8 days, on intermediate leaves, of the seagrass Cymodocea nodosa. Hydrogen peroxide (H₂O₂) production, lipid peroxidation, protein, phenolic content and antioxidant enzyme activities were investigated. Increased H₂O₂ formation was detected even at the lowest BPA treatments from the beginning of the experiment and both the enzymatic and non-enzymatic antioxidant defense mechanisms were activated upon application of BPA. Elevated H₂O₂ levels that were detected as a response to increasing BPA concentrations and incubation time, led to the decrease of protein content on the 4th day even at the two lower BPA concentrations, and to the increase of the lipid peroxidation at the highest concentration. However, on the 6th day of BPA exposure, protein content did not differ from the control, indicating the ability of both the enzymatic and non-enzymatic mechanisms (such as superoxide dismutase (SOD) and phenolics) to counteract the BPA-derived oxidative stress. The early response of the protein content determined that the Low Effect Concentration (LOEC) of BPA is 0.3 μg L⁻¹ and that the protein content meets the requirements to be considered as a possible early warning “biomarker” for C. nodosa against BPA toxicity.

【 授权许可】

Unknown