A two-step species-specific 16S rRNA PCR assay for the detection of Taylorella equigenitalis in horses

Irish Veterinary Journal

关键词: Horse;    Taylorella equigenitalis;    16S rRNA;    Contagious equine metritis;    Polymerase chain reaction;   

DOI  :  10.1186/2046-0481-58-3-146

来源: DOAJ

【 摘 要 】

A two-step PCR assay was developed for the molecular detection of Taylorella equigenitalis, a Gram-negative genital bacterial pathogen in horses. Two specific oligonucleotide primers (TE16SrRNABCHf [25mer] and TE16SrRNABCHr [29mer]) were designed from multiple alignments of the 16S rRNA gene loci of several closely related taxa, including T. asinigenitalis. Subsequent enhanced surveillance of 250 Thoroughbred animals failed to detect the presence of this organism directly from clinical swabs taken from the genital tract of mares and stallions. Such a molecular approach offers a sensitive and specific alternative to conventional culture techniques, and has the potential to lead to improved diagnosis and subsequent management of horses involved in breeding programmes.

【 授权许可】

Unknown