Survival of taylorellae in the environmental amoeba Acanthamoeba castellanii

BMC Microbiology

Laurent Hébert²  Sandrine Petry²  Claire Laugier²  Anne Vianney¹  Julie Allombert¹ 

[1] CNRS, UMR5308, Lyon, France;ANSES, Dozulé Laboratory for Equine Diseases, Bacteriology and Parasitology Unit, 14430 Goustranville, France

关键词: Endosymbiont;    Acanthamoeba castellanii;    Contagious equine metritis;    Taylorella asinigenitalis;    Taylorella equigenitalis;   

Others  :  1141647
DOI  :  10.1186/1471-2180-14-69

 received in 2014-01-27, accepted in 2014-03-13,  发布年份 2014

【 摘 要 】

Background

Taylorella equigenitalis is the causative agent of contagious equine metritis, a sexually-transmitted infection of Equidae characterised in infected mares by abundant mucopurulent vaginal discharge and a variable degree of vaginitis, cervicitis or endometritis, usually resulting in temporary infertility. The second species of the Taylorella genus, Taylorella asinigenitalis, is considered non-pathogenic, although mares experimentally infected with this bacterium can develop clinical signs of endometritis. To date, little is understood about the basic molecular virulence and persistence mechanisms employed by the Taylorella species. To clarify these points, we investigated whether the host-pathogen interaction model Acanthamoeba castellanii was a suitable model for studying taylorellae.

Results

We herein demonstrate that both species of the Taylorella genus are internalised by a mechanism involving the phagocytic capacity of the amoeba and are able to survive for at least one week inside the amoeba. During this one-week incubation period, taylorellae concentrations remain strikingly constant and no overt toxicity to amoeba cells was observed.

Conclusions

This study provides the first evidence of the capacity of taylorellae to survive in a natural environment other than the mammalian genital tract, and shows that the alternative infection model, A. castellanii, constitutes a relevant alternative system to assess host-pathogen interactions of taylorellae. The survival of taylorellae inside the potential environmental reservoir A. castellanii brings new insight, fostering a broader understanding of taylorellae biology and its potential natural ecological niche.

【 授权许可】

   
2014 Allombert et al.; licensee BioMed Central Ltd.

【 图 表 】

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