Green synthesis of silver nanoparticles using Citrus limon peels and evaluation of their antibacterial and cytotoxic properties

Saudi Journal of Biological Sciences

Ashraf A. Hatamleh¹  Nouf M. Al-Enazi²  Noura S. Aldosari³  Manal M. Alkhulaifi³  Moudi A. Alwehaibi³  Jamilah H. Alshehri³  Neveen Abdel- Raouf³  Manal A. Awad⁴ 

[1] Corresponding authors at: Department of Biology, College of Science and Humanities in Al-Kharj, Prince Sattam bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia (N. Abdel-Raouf).;Department of Biology, College of Science and Humanities in Al-Kharj, Prince Sattam bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia;Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;King Abdullah Institute for Nanotechnology, King Saud University, Saudi Arabia;

关键词: Green Silver nanoparticles;    Citrus limon peels;    Antibacterial activity;    Cytotoxic activity;    Breast cancer;    Carcinoma cell line;   

DOI  :  

来源: DOAJ

【 摘 要 】

The present work aimed to synthesis silver nanoparticles (AgNPs) using biological waste products Citrus limon peels, its characterization, antimicrobial activities and the cytotoxic effect of the synthesized green AgNPs. Characterization of the prepared AgNPs showed the formation of spherical, and few agglomerated AgNPs forms as measured by UV–visible spectrophotometer. The average size of the prepared AgNPs was 59.74 nm as measured by DLS technique. The spectrum of the synthesized AgNPs was observed at 3 KeV using the EDX. On the other hand, FTIR analysis of the green synthesized AgNPs showed the presence of alcohols, phenolics, mono-substituted alkynes, aliphatic primary amines, sodium salt, amino acid, or SiOH alcohol groups. The antimicrobial studies of the formed AgNPs showed positive activity against most of the studied human pathogenic bacteria with varying degrees. Finally, the evaluation of the cytotoxic effect of the green synthesized AgNPs were done using two types of cell lines, human breast cancer cell line (MCF-7) and human colon carcinoma cell line (HCT-116). The results revealed the concentration has a direct correlation with cell viability. The 50% inhibitory concentration (IC50) of MCF-7 cell line was in of 23.5 ± 0.97 µL/100 µL, whereas the HCT-116 cell line was in 37.48 ± 5.93 µL/100 µL.

【 授权许可】

Unknown