Afamin Levels and Their Correlation with Oxidative and Lipid Parameters in Non-diabetic, Obese Patients

Biomolecules

Sándor Somodi²  Imre Juhász²  Mariann Harangi³  Ildikó Seres³  György Paragh³  Viktória Evelin Varga³  Hajnalka Lőrincz³  Szilvia Ujfalusi⁴ 

[1] Department of Dermatology, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14203, USA;Department of Emergency Medicine, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary;Department of Internal Medicine, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary;Doctoral School of Health Sciences, Faculty of Public Health, University of Debrecen, 4032 Debrecen, Hungary;

关键词: obesity;    insulin resistance;    afamin;    HDL subfractions;    vitamin E;   

DOI  :  10.3390/biom12010116

来源: DOAJ

【 摘 要 】

Background: Afamin is a liver-produced bioactive protein and features α- and γ-tocopherol binding sites. Afamin levels are elevated in metabolic syndrome and obesity and correlate well with components of metabolic syndrome. Afamin concentrations, correlations between afamin and vitamin E, afamin and lipoprotein subfractions in non-diabetic, obese patients have not been fully examined. Methods: Fifty non-diabetic, morbidly obese patients and thirty-two healthy, normal-weight individuals were involved in our study. The afamin concentrations were measured by ELISA. Lipoprotein subfractions were determined with gel electrophoresis. Gas chromatography–mass spectrometry was used to measure α- and γ tocopherol levels. Results: Afamin concentrations were significantly higher in the obese patients compared to the healthy control (70.4 ± 12.8 vs. 47.6 ± 8.5 μg/mL, p < 0.001). Positive correlations were found between afamin and fasting glucose, HbA1c, hsCRP, triglyceride, and oxidized LDL level, as well as the amount and ratio of small HDL subfractions. Negative correlations were observed between afamin and mean LDL size, as well as the amount and ratio of large HDL subfractions. After multiple regression analysis, HbA1c levels and small HDL turned out to be independent predictors of afamin. Conclusions: Afamin may be involved in the development of obesity-related oxidative stress via the development of insulin resistance and not by affecting α- and γ-tocopherol levels.

【 授权许可】

Unknown