Biosynthesis of Polyhydroxyalkanoates (PHAs) by the Valorization of Biomass and Synthetic Waste

Molecules

Naveeda Riaz¹  Hooria Khan²  Imdad Kaleem²  Kaleem Ullah³  Ghulam Murtaza⁴  Robina Manzoor⁵  Aamir Rasool⁶  Hamid Mukhtar⁷  SyedaMichelle Naqvi⁷  Sheeba Shakoor⁷  Hadiqa Javaid⁷  Ikram -Ul-Haq⁷  KanitaAhmed Shah⁷  Ali Nawaz⁷ 

[1] Department of Biological Sciences, International Islamic University, Islamabad 45550, Pakistan;Department of Biosciences, COMSATS University Islamabad (CUI), Islamabad 45550, Pakistan;Department of Microbiology, University of Balochistan, Quetta 87300, Pakistan;Department of Zoology, University of Gujrat, Gujrat 50700, Pakistan;Faculty of Marine Sciences, Lasbella University of Agriculture, Water and Marine Sciences, Balochistan 90150, Pakistan;Institute of Biochemistry, University of Balochistan, Quetta 87300, Pakistan;Institute of Industrial Biotechnology (IIB), Government College University, Lahore 54000, Pakistan;

关键词: biomass valorization;    biodegradation;    biopolymer;    biomaterials;    bioplastic;    plastic bag;   

DOI  :  10.3390/molecules25235539

来源: DOAJ

【 摘 要 】

Synthetic pollutants are a looming threat to the entire ecosystem, including wildlife, the environment, and human health. Polyhydroxyalkanoates (PHAs) are natural biodegradable microbial polymers with a promising potential to replace synthetic plastics. This research is focused on devising a sustainable approach to produce PHAs by a new microbial strain using untreated synthetic plastics and lignocellulosic biomass. For experiments, 47 soil samples and 18 effluent samples were collected from various areas of Punjab, Pakistan. The samples were primarily screened for PHA detection on agar medium containing Nile blue A stain. The PHA positive bacterial isolates showed prominent orange–yellow fluorescence on irradiation with UV light. They were further screened for PHA estimation by submerged fermentation in the culture broth. Bacterial isolate 16a produced maximum PHA and was identified by 16S rRNA sequencing. It was identified as Stenotrophomonas maltophilia HA-16 (MN240936), reported first time for PHA production. Basic fermentation parameters, such as incubation time, temperature, and pH were optimized for PHA production. Wood chips, cardboard cutouts, plastic bottle cutouts, shredded polystyrene cups, and plastic bags were optimized as alternative sustainable carbon sources for the production of PHAs. A vital finding of this study was the yield obtained by using plastic bags, i.e., 68.24 ± 0.27%. The effective use of plastic and lignocellulosic waste in the cultivation medium for the microbial production of PHA by a novel bacterial strain is discussed in the current study.

【 授权许可】

Unknown