Evaluation of an Ionic Calcium Fiber Supplement and Its Impact on Bone Health Preservation in a Dietary Calcium Deficiency Mice Model

Nutrients

Eduardo Padilla-Camberos¹  Hugo Espinosa-Andrews²  Eristeo García-Márquez³  Sara Elisa Herrera-Rodríguez⁴ 

[1] Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Av. Normalistas 800, Colinas de La Normal, Guadalajara 44270, Jalisco, Mexico;Tecnología Alimentaria, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., CIATEJ, Cam. Arenero 1227, El Bajío, Zapopan 45019, Jalisco, Mexico;Unidad Noreste, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Autopista Mty-Aeropuerto, Vía de la Innovación 404, Parque PIIT, Cd Apodaca 66628, Nuevo León, Mexico;Unidad Sureste, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Km 5.5 Carretera, Sierra Papacal-Chuburná, Chuburná, Mérida 97302, Yucatán, Mexico;

关键词: ionic calcium;    fiber supplementation;    osteoporosis;    calcium-free diet;   

DOI  :  10.3390/nu14030422

来源: DOAJ

【 摘 要 】

Ionic calcium can help in the prevention of the process of osseous decalcification. This study aimed to evaluate the physicochemical properties and toxic effects of ionic calcium-fiber supplement (ICa⁺) and its impact on bone health preservation in mice C57/BL6 fed a calcium-deficient diet. Physicochemical properties include FTIR, apparent calcium solubility estimated by the calcium ratio obtained by ionization chromatography and atomic absorption. In vitro genotoxicity and cytotoxicity of the ICa⁺ were assessed. Twenty-five 7-week-old C57/BL6 mice were fed calcium-free diet (CFD) or CFD plus CaCO₃ (1.33 mg Ca) or CFD plus ICa⁺ (1.33–6.66 mg Ca) for six weeks. After that, bone mass and microstructure parameters were assessed. Histological staining was performed to determine calcium deposits. ICa⁺ (100%) exhibited an apparent calcium solubility higher than CaCO₃ (12.3%). ICa⁺ showed no cytotoxic and genotoxic in vitro activities. Histomorphometry analysis showed that the ICa⁺ treated group displayed a higher trabecular number than the trabecular space. Also, the ratio BV/TV was increased compared with all treatments. Ionic calcium-fiber supplementation prevents bone deterioration compared to mice fed a calcium-deficient diet.

【 授权许可】

Unknown