Use of iQPR-H2O for bone regeneration and its potential in the improvement of osteoporosis

BMC Musculoskeletal Disorders

Winping Deng⁶  Gowlin Lam⁶  Kwanghwa Chang⁷  Chihhsiung Wu³  Mingfu Wang²  Chingyu Tsai⁶  Henyu Liu¹  Wentien Hsiao⁵  Meileng Cheong⁴  Chiming Lee⁵ 

[1] Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan;Department of Food and Nutrition, Providence University, Taichung, Taiwan;Department of Surgery, School of Medicine, Taipei Medical University-Shuang Ho Hospital, Taipei, Taiwan;Department of Obstetrics and Gynecology, Cathay General Hospital, Taipei, Taiwan;Department of Radiology, Taipei Medical University Hospital, Taipei, Taiwan;Graduate Institute of Biomedical Materials and Engineering Taipei Medical University, Taipei, Taiwan;Department of Physical Medicine and Rehabilitation, Wan Fang Hospital, Taipei Medical University, Taiwan

关键词: bone mass density;    senescence-accelerated mice;    mouse fibroblasts;    Quantum Persistent Reflection;    osteoporosis;   

Others  :  1151903
DOI  :  10.1186/1471-2474-12-227

 received in 2010-12-31, accepted in 2011-10-08,  发布年份 2011

【 摘 要 】

Background

Current treatments for osteoporosis are associated with various side effects and do not prevent the age-related decrease in osteoblast number. The objective of this study was to evaluate the effects of iQPR-H₂O on osteogenesis.

Methods

Mouse fibroblast NIH3T3 and pre-osteoblastic MC3T3-E1 cells were cultured in medium prepared with iQPR-H₂O or unprocessed mineral water (control cells), and proliferation and differentiation were assessed by MTT and alkaline phosphatase assay, respectively. Mineral deposition by the cells was determined using Alizarin red S staining. A mouse model of osteoporosis, ovariectomized SAMP8 mice, was used to evaluate the effects of iQPR-H₂O on osteogenesis in vivo. Mice were given either iQPR-H₂O or unprocessed mineral water (control group) for four months after which bone mass density (BMD) measurements were made using a bone densitometer and hematoxylin and eosin staining of bone samples.

Results

NIH3T3 cells grown in medium prepared with iQPR-H₂O exhibited significantly greater proliferation. NIH3T3 and MC3T3-E1 cells demonstrated a significant increase in alkaline phosphatase levels in the iQPR-H₂O group. MC3T3-E1 cells showed mineralization at day 28. mRNA expression levels of both osteopontin and runt-related transcription factor 2 in MC3T3-E1 cells were higher in the iQPR-H₂O group compared with the control group. After four months, significantly greater bone regeneration was evident in ovariectomized SAMP8 mice administered iQPR-H₂O as compared with control group.

Conclusions

iQPR-H₂O may reduce the symptoms of osteoporosis by improving osteogenesis.

【 授权许可】

   
2011 Lee et al; licensee BioMed Central Ltd.

【 图 表 】

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