Classification of Various Marijuana Varieties by Raman Microscopy and Chemometrics

Toxics

Luis Ramos-Guerrero¹  Marzia Cosmi²  Gemma Montalvo³  Fernando E. Ortega-Ojeda³  Carmen García-Ruiz³ 

[1] Centro de Investigación de Alimentos, CIAL—Centro de Investigación de Alimentos, Universidad UTE, Quito EC170527, Ecuador;Department of Engineering and Architecture, University of Trieste Via Alfonso Valerio 6a, 34127 Trieste, Italy;Universidad de Alcalá, Departamento de Química Analítica, Química Física e Ingeniería Química, Ctra. Madrid–Barcelona km 33,600, 28871 Alcalá de Henares, Madrid, Spain;

关键词: marijuana;    trichome;    chemometrics;    Raman microscopy;    discrimination;    OPLS-DA;   

DOI  :  10.3390/toxics10030115

来源: DOAJ

【 摘 要 】

The Raman analysis of marijuana is challenging because of the sample’s easy photo-degradation caused by the laser intensity. In this study, optimization of collection parameters and laser focusing on marijuana trichome heads allowed collecting Raman spectra without damaging the samples. The Raman spectra of Δ⁹-tetrahydrocannabinol (THC), cannabidiol (CBD), and cannabinol (CBN) standard cannabinoids were compared with Raman spectra of five different types of marijuana: four Sativa varieties (Amnesia Haze, Amnesia Hy-Pro, Original Amnesia, and Y Griega) and one Indica variety (Black Domina). The results verified the presence of several common spectral bands that are useful for marijuana characterization. Results were corroborated by the quantum chemical simulated Raman spectra of their acid-form (tetrahydrocannabinol acid (THCA), cannabidiol acid (CBDA)) and decarboxylated cannabinoids (THC, CBD, and CBN). A chemometrics-assisted method based on Raman microscopy and OPLS-DA offered good classification among the different marijuana varieties allowing identification of the most significant spectral bands.

【 授权许可】

Unknown