Effects of three graphene-based materials on the growth and photosynthesis of Brassica napus L.

Ecotoxicology and Environmental Safety

Xiuping Wang¹  Aihua Sha²  Jun Li³  Chang Chen³  Lixin Liu³  Xiaolu Xiao³ 

[1] Analysis and Testing Center, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China;Hubei Collaborative Innovation Center for Grain Industry, Yangtze University, Jingzhou 434023, China;Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Science, Wuhan 430062, China;

关键词: Reduced graphene oxide;    Graphene oxide;    Amine-functionalized graphene;    Phytotoxicity;    Photosynthesis;   

DOI  :  

来源: DOAJ

【 摘 要 】

The environmental safety and threats of graphene-based materials (GBMs) to the agroecosystem have attracted increasing attention in recent years. However, the mechanisms underlying the effects of GBMs on plants remain unclear. Here, we investigated the phytotoxicity of reduced graphene oxide (RGO), graphene oxide (GO) and amine-functionalized graphene (G-NH2) on Brassica napus L. The results revealed that RGO impaired photosynthesis mainly by decreasing the chlorophyll content and Rubisco activity. A further gene-level analysis suggested that this effect of RGO might be due to its toxicity on sulfate transmembrane transporter and nitrogen metabolism, which ultimately led to nutrient imbalance. However, GO directly damaged the photosystem by disrupting the chloroplast structure, and a decrease in Rubisco activity indicated that GO also inhibits carbon fixation. Further gene-level analysis demonstrated that GO has toxicity on the chloroplast membrane, photosystem, photosynthethic electron transport and F-type ATPase. In addition, G-NH2 at 10–1000 mg L−1 showed no significant toxicity. These findings shed light on the potential mechanism for the toxicity of GBMs on plants for risk assessment.

【 授权许可】

Unknown