Aberrant Expression of Long Non-coding RNAs in Exosomes in Follicle Fluid From PCOS Patients

Frontiers in Genetics

Hairui Fan¹  Qiuyue Yuan²  Xiaomei Zhang³  Yinggang Zou⁴  Hengmi Cui⁵  Xuming Hu⁵  Xujing Chen⁵  Chunhui Zhu⁵  Liping Wang⁵ 

[1] College of Animal Science and Technology, Yangzhou University, Yangzhou, China;College of Pharmacy, Dalian Medical University, Dalian, China;Department of Biobank Clinical Medical College, Yangzhou University, Yangzhou, China;Department of Obstetrics and Gynecology, The Second Hospital of Jilin University, Changchun, China;Institute of Epigenetics and Epigenomics, College of Animal Science and Technology, Yangzhou University, Yangzhou, China;

关键词: polycystic ovary syndrome;    follicle fluid;    exosomes;    long non-coding RNAs;    bioinformatics;   

DOI  :  10.3389/fgene.2020.608178

来源: DOAJ

【 摘 要 】

Polycystic ovary syndrome (PCOS) is a common reproductive endocrine disease characterized by persistent anovulation and hyperandrogenism, affecting approximately 8–10% of women of childbearing age and occupying an important position in the etiology of infertility. There is increasing evidence that long non-coding RNAs (lncRNAs) are involved in the development of PCOS, but the potential regulatory mechanism is still unclear. This study performed high-throughput lncRNA sequencing of follicular fluid exosomes in non-PCOS infertility patients and PCOS infertility patients. The sequencing results led to the identification of 1,253 upregulated and 613 downregulated lncRNAs from a total of 1,866 detected candidates. There was no significant difference between the PCOS patients and non-PCOS patients in body mass index (BMI) or the fasting blood glucose (FBG) level. However, luteinizing hormone (LH), estradiol (E2), testosterone (T), serum prolactin (PRL), and anti-Mullerian hormone (AMH) levels were clearly upregulated in PCOS patients compared to those in non-PCOS patients. There was also an increase in LH/FSH (>2) in the PCOS patients. Functional analysis showed pathways related to endocytosis, the Hippo, the MAPK, and HTLV-1 infection. These results suggest that lncRNAs may play an important role in the pathogenesis of PCOS and may be potential targets for the diagnosis and treatment of PCOS.

【 授权许可】

Unknown