Bone Marrow-Derived lin−c-kit+Sca-1+ Stem Cells Do Not Contribute to Vasculogenesis in Lewis Lung Carcinoma

Neoplasia: An International Journal for Oncology Research

Erik B. Friedrich¹  Robert E. Gerszten¹  Allison E. Wolley²  Jennifer R. Allport²  Vivek R. Shinde Patil²  Ralph Weissleder² 

[1] Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA;Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA;

关键词: Stem cells;    vasculogenesis;    angiogenesis;    tumors;    chemokine;   

DOI  :  10.1593/neo.04523

来源: DOAJ

【 摘 要 】

The development of tumor vasculature is thought to occur through two complementary processes: sprouting angiogenesis from preexisting blood vessels of the host, vasculogenesis, which involves the spontaneous development of vessels through specific recruitment, differentiation, vascular incorporation of circulating endothelial cells (EC), endothelial progenitor cells (EPC), or potentially bone marrow-derived cells. Recent reports, however, have challenged the belief that bone marrow-derived cells contribute to tumor neovascularization, claiming an exclusive role for sprouting angiogenesis in tumor blood vessel development. In the present study, we explored the recruitment behavior of bone marrow-derived lin− c-kit+Sca-1+ stem cells to subcutaneously implanted Lewis lung carcinoma in a syngeneic bone marrow transplantation model. We observed that although lin− c-kit+Sca-1+ and their derived cells demonstrate significant recruitment to carcinomas in vivo, they do not appear to functionally contribute to tumor neovascularization. Furthermore, our results support the hypothesis that new vessel formation in carcinomas occurs primarily through endothelialization from adjacent and preexisting vasculature.

【 授权许可】

Unknown