Metal Nanoparticles Released from Dental Implant Surfaces: Potential Contribution to Chronic Inflammation and Peri-Implant Bone Loss

Materials

Cristian Taccioli¹  Luca Sbricoli²  Giulia Brunello²  Chiara Gardin²  Eitan Mijiritsky³  Eriberto Bressan³  Devorah Schwartz-Arad³  Miguel Penarrocha³  Marco Tatullo⁴  Gloria Bellin⁵  Letizia Ferroni⁵  Stefano Sivolella⁵  Barbara Zavan⁶  Adriano Piattelli⁶  David Penarrocha⁷ 

[1] Endodontology, 5219100 Ramat Hasharon, Israel;Research, 48033 Cotignola (RA), Italy;Department of Neurosciences, University of Padova, 35133 Padova, Italy;Deptartment of Otolaryngology, Head and Neck and Maxillofacial Surgery, Tel-Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel-Aviv University, 5219100 Ramat Aviv, Israel;;Maria Cecilia Hospital, GVM Care &Oral Surgery and Implantology, Valencia University Medical and Dental School, 46004 Valencia, Spain;;Schwartz-Arad Day-Care Surgical Center, Maxillo-Facial Surgery, Advanced Implantology, Periodontology &

关键词: titanium nanoparticles;    reactive oxygen species;    osteolysis;   

DOI  :  10.3390/ma12122036

来源: DOAJ

【 摘 要 】

Peri-implantitis is an inflammatory disease affecting tissues surrounding dental implants. Although it represents a common complication of dental implant treatments, the underlying mechanisms have not yet been fully described. The aim of this study is to identify the role of titanium nanoparticles released form the implants on the chronic inflammation and bone lysis in the surrounding tissue. We analyzed the in vitro effect of titanium (Ti) particle exposure on mesenchymal stem cells (MSCs) and fibroblasts (FU), evaluating cell proliferation by MTT test and the generation of reactive oxygen species (ROS). Subsequently, in vivo analysis of peri-implant Ti particle distribution, histological, and molecular analyses were performed. Ti particles led to a time-dependent decrease in cell viability and increase in ROS production in both MSCs and FU. Tissue analyses revealed presence of oxidative stress, high extracellular and intracellular Ti levels and imbalanced bone turnover. High expression of ZFP467 and the presence of adipose-like tissue suggested dysregulation of the MSC population; alterations in vessel morphology were identified. The results suggest that Ti particles may induce the production of high ROS levels, recruiting abnormal quantity of neutrophils able to produce high level of metalloproteinase. This induces the degradation of collagen fibers. These events may influence MSC commitment, with an imbalance of bone regeneration.

【 授权许可】

Unknown