Regulatory impairment in untreated Parkinson’s disease is not restricted to Tregs: other regulatory populations are also involved

Journal of Neuroinflammation

Edgar Sevilla-Reyes¹  Edda Sciutto²  Gladis Fragoso²  Ulises Rodríguez-Ortiz³  Marie Catherine Boll³  Diana D. Álvarez-Luquín⁴  Esteban Montes-Moratilla⁴  Raquel Espinoza-Cárdenas⁴  Jaquelín Leyva-Hernández⁴  Asiel Arce-Sillas⁴  Laura Adalid-Peralta⁴  Viridiana Vivas-Almazán⁴  Citzielli Pérez-Correa⁴ 

[1] Clinica de Investigación en Enfermedades Infecciosas (CIENI), Instituto Nacional de Enfermedades Respiratorias;Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México;Instituto Nacional de Neurología y Neurocirugía;Unidad Periférica para el Estudio de la Neuroinflamación en Patologías Neurológicas del Instituto de Investigaciones Biomédicas en el Instituto Nacional de Neurología y Neurocirugía;

关键词: Parkinson’s disease;    Untreated patients;    Tregs;    Bregs;    CD8regs;    Peripheral immune response;   

DOI  :  10.1186/s12974-019-1606-1

来源: DOAJ

【 摘 要 】

Abstract Background Parkinson’s disease (PD) is the second most common neurodegenerative disease in the world. Various studies have suggested that the immune response plays a key role in this pathology. While a predominantly pro-inflammatory peripheral immune response has been reported in treated and untreated PD patients, the study of the role of the regulatory immune response has been restricted to regulatory T cells. Other immune suppressive populations have been described recently, but their role in PD is still unknown. This study was designed to analyze the pro and anti-inflammatory immune response in untreated PD patients, with emphasis on the regulatory response. Methods Thirty-two PD untreated patients and 20 healthy individuals were included in this study. Peripheral regulatory cells (CD4+Tregs, Bregs, CD8+Tregs, and tolerogenic dendritic cells), pro-inflammatory cells (Th1, Th2, and Th17 cells; active dendritic cells), and classical, intermediate, and non-classical monocytes were characterized by flow cytometry. Plasmatic levels of TNF-α, IFN-γ, IL-6, GM-CSF, IL-12p70, IL-4, IL-13, IL-17α, IL-1β, IL-10, TGF-β, and IL-35 were determined by ELISA. Results Decreased levels of suppressor Tregs, active Tregs, Tr1 cells, IL-10-producer CD8regs, and tolerogenic PD-L1+ dendritic cells were observed. With respect to the pro-inflammatory response, a decrease in IL-17-α and an increase in IL-13 levels were observed. Conclusion A decrease in the levels of regulatory cell subpopulations in untreated PD patients is reported for the first time in this work. These results suggest that PD patients may exhibit a deficient suppression of the pro-inflammatory response, which could contribute to the pathophysiology of the disease.

【 授权许可】

Unknown