Staphylococcus aureus with inducible clindamycin resistance and methicillin resistance in a tertiary hospital in Nepal

Tropical Medicine and Health

Nabaraj Adhikari¹  Mahesh Chaudhari²  Sabita Thapa³  Devi Thapa³  Suresh Lamsal³  Dhiraj Shrestha⁴  Susil Pyakurel⁴ 

[1] Central Department of Microbiology, Tribhuvan University;Department of Microbiology, KIST Medical College;Department of Microbiology, Kantipur College of Medical Science;Department of Microbiology, Shi-Gan International College of Science and Technology;

关键词: Inducible clindamycin resistance;    MRSA;    Nepal;    S. aureus;   

DOI  :  10.1186/s41182-021-00392-2

来源: DOAJ

【 摘 要 】

Abstract Background Staphylococcus aureus is a global public health issue in both community and hospital settings. Management of methicillin-resistant S. aureus (MRSA) infections are tough owing to its resistance to many antibiotics. Macrolide-lincosamide-streptogramin B (MLSB) antibiotics are commonly used for the management of MRSA. This study was aimed to determine the occurrence of inducible clindamycin- and methicillin-resistant S. aureus at a tertiary care hospital in Kathmandu, Nepal. Methods A total of 1027 clinical samples were processed following standard laboratory procedures and antibiotic susceptibility testing of S. aureus was performed by disc diffusion method. MRSA isolates were detected phenotypically using cefoxitin disc, and inducible clindamycin resistance was detected phenotypically using the D-zone test. Results Of 1027 samples, 321 (31.2%) were culture positive, of which 38 (11.8%) were S. aureus. All S. aureus isolates were susceptible to vancomycin, and 25 (67%) of S. aureus isolates were multidrug-resistant. Similarly, 15 (39.5%) of S. aureus were MRSA and 14 (36.5%) were inducible clindamycin-resistant phenotypes. Conclusion Inducible clindamycin and methicillin resistance were common in S. aureus. This emphasizes that the methicillin resistance test and the D-zone test should be incorporated into the routine antibiotic susceptibility testing in hospital settings.

【 授权许可】

Unknown