Inhibition of TYRO3/Akt signaling participates in hypoxic injury in hippocampal neurons

Neural Regeneration Research

关键词: nerve regeneration;    TYRO3;    Akt;    apoptosis;    hippocampal neurons;    primary culture;    hypoxia;    proliferation;    neural regeneration;   

DOI  :  10.4103/1673-5374.182701

来源: DOAJ

【 摘 要 】

In this study, we investigated the role of the TYRO3/Akt signaling pathway in hypoxic injury to hippocampal neurons. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that hypoxia inhibited the proliferation and viability of hippocampal neurons. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay demonstrated that hypoxia induced neuronal apoptosis in a time-dependent manner, with a greater number of apoptotic cells with longer hypoxic exposure. Immunofluorescence labeling revealed that hypoxia suppressed TYRO3 expression. Western blot assay showed that hypoxia decreased Akt phosphorylation levels in a time-dependent manner. Taken together, these findings suggest that hypoxia inhibits the proliferation of hippocampal neurons and promotes apoptosis, and that the inhibition of the TYRO3/Akt signaling pathway plays an important role in hypoxia-induced neuronal injury.

【 授权许可】

Unknown