Resveratrol Ameliorates Microcystin-LR-Induced Testis Germ Cell Apoptosis in Rats via SIRT1 Signaling Pathway Activation

Toxins

Yueqin Wang¹  Xingde Du¹  Haohao Liu¹  Jinxia Wu¹  Donggang Zhuang¹  Huizhen Zhang¹  Shenshen Zhang¹  PhelistersWegesa Marwa¹  Xuemin Cheng¹  Chuanrui Liu¹  Rui Wang¹  Le Yuan¹ 

[1] College of Public Health, Zhengzhou University, Zhengzhou 450001, China;

关键词: apoptosis;    microcystin-LR (MC-LR);    reproductive toxicity;    resveratrol;    sirtuin 1 (SIRT1);   

DOI  :  10.3390/toxins10060235

来源: DOAJ

【 摘 要 】

Microcystin-leucine arginine (MC-LR), a cyclic heptapeptide produced by cyanobacteria, is a strong reproductive toxin. Studies performed in rat Sertoli cells and Chinese hamster ovary cells have demonstrated typical apoptosis after MC-LR exposure. However, little is known on how to protect against the reproductive toxicity induced by MC-LR. The present study aimed to explore the possible molecular mechanism underlying the anti-apoptosis and protective effects of resveratrol (RES) on the co-culture of Sertoli–germ cells and rat testes. The results demonstrated that MC-LR treatment inhibited the proliferation of Sertoli–germ cells and induced apoptosis. Furthermore, sirtuin 1 (SIRT1) and Bcl-2 were inhibited, while p53 and Ku70 acetylation, Bax expression, and cleaved caspase-3 were upregulated by MC-LR. However, RES pretreatment ameliorated MC-LR-induced apoptosis and SIRT1 inhibition, and downregulated the MC-LR-induced increase in p53 and Ku70 acetylation, Bax expression, and caspase-3 activation. In addition, RES reversed the MC-LR-mediated reduction in Ku70 binding to Bax. The present study indicated that the administration of RES could ameliorate MC-LR-induced Sertoli–germ cell apoptosis and protect against reproductive toxicity in rats by stimulating the SIRT1/p53 pathway, suppressing p53 and Ku70 acetylation and enhancing the binding of Ku70 to Bax.

【 授权许可】

Unknown