Comparative analysis of high altitude hypoxia induced erythropoiesis and iron homeostasis in Indian and Kyrgyz lowlander males

Current Research in Biotechnology

Akpay Sarybaev¹  Priya Gaur²  Supriya Saini²  Yogendra Kumar Sharma²  Bhuvnesh Kumar²  Sanjeev Kumar Sharma²  Koushik Ray²  Krishna Kishore²  Kushubakova Nadira Asanbekovna³  Almaz Akunov³  Abdirashit Maripov³  Praveen Vats³  Shashi Bala Singh⁴ 

[1] Research, Hyderabad, Telangana 500037, India;Defence Institute of Physiology and Allied Sciences, Lucknow Road, Timarpur, Delhi 110054, India;Kyrgyz Indian Mountain Biomedical Research Centre, Togolok Moldo Str 3, 720040 Bishkek, Kyrgyzstan;;National Institute of Pharmaceutical Education &

关键词: High altitude;    Ethnicity;    RNA sequencing;    Erythropoiesis;    Hepcidin;    Acclimatization;   

DOI  :  

来源: DOAJ

【 摘 要 】

Lowland residents acclimatize to reduced oxygen availability at high altitude by certain functional alterations in body which vary according to the different lifestyle, geographical location, ethnicity etc. In our study, two different ethnic lowlander groups acclimatization response was investigated during high altitude exposure. Overnight fasting blood of Indian (n = 20) and Kyrgyz (n = 20) at baseline and high altitude (4,111 m) day 3, 7, 14, 21 was collected and analysed. High altitude induced erythropoiesis was higher in Indians compared to Kyrgyz. Hemoglobin, hematocrit, red blood cells increased till high altitude day 21 and erythropoietin till day 7 in Indians. Hepcidin decrease was double in Indians compared with Kyrgyz and iron, ferritin and transferrin receptor were found synchronized to maintain circulatory iron for erythropoiesis. Further to validate, RNA sequencing analysis of differentially expressed gene by DAVID hit hematopoietic cell lineage among top up regulated pathway in Indian vs Kyrgyz. IPA analysis disclosed GATA1 as second most top upstream regulator in Indians as compared to Kyrgyz at high altitude day7 and activation of haematological system development and function at HAD14 in Indian vs Kyrgyz. Valedictory qRT-PCR for putative genes like HAMP, BMP6, EPOR, etc. indicated differences in erythroid and iron regulation in both. Our data strongly reflected different erythropoietic response towards altitude exposure in two different ethnic lowlanders.

【 授权许可】

Unknown