Common Metabolites in Two Different Hypertensive Mouse Models: A Serum and Urine Metabolome Study

Biomolecules

Cristina M. Arenaz¹  Aja K. Coleman¹  Brett M. Mitchell¹  Bethany L. Goodlett¹  Gaurav Baranwal¹  Joseph M. Rutkowski¹  Arul Jayaraman²  Robert C. Alaniz²  Rachel Pilla³ 

[1] Department of Medical Physiology, College of Medicine, Texas A&M University, Bryan, TX 77847, USA;Department of Microbial Pathogenesis and Immunology, College of Medicine, Texas A&M University, Bryan, TX 77847, USA;Department of Small Animal Clinical Science, College of Veterinary Medicine & Biomedical Science, Texas A&M University, College Station, TX 77843, USA;

关键词: microbial metabolites;    angiotensin II;    salt;    hypertension;    metabolomics;   

DOI  :  10.3390/biom11091387

来源: DOAJ

【 摘 要 】

Recent metabolomics studies have identified a wide array of microbial metabolites and metabolite pathways that are significantly altered in hypertension. However, whether these metabolites play an active role in pathogenesis of hypertension or are altered because of this has yet to be determined. In the current study, we hypothesized that metabolite changes common between hypertension models may unify hypertension’s pathophysiology with respect to metabolites. We utilized two common mouse models of experimental hypertension: L-arginine methyl ester hydrochloride (L-NAME)/high-salt-diet-induced hypertension (LSHTN) and angiotensin II induced hypertension (AHTN). To identify common metabolites that were altered across both models, we performed untargeted global metabolomics analysis in serum and urine and the resulting data were analyzed using MetaboAnalyst software and compared to control mice. A total of 41 serum metabolites were identified as being significantly altered in any hypertensive model compared to the controls. Of these compounds, 14 were commonly changed in both hypertensive groups, with 4 significantly increased and 10 significantly decreased. In the urine, six metabolites were significantly altered in any hypertensive group with respect to the control; however, none of them were common between the hypertensive groups. These findings demonstrate that a modest, but potentially important, number of serum metabolites are commonly altered between experimental hypertension models. Further studies of the newly identified metabolites from this untargeted metabolomics analysis may lead to a greater understanding of the association between gut dysbiosis and hypertension.

【 授权许可】

Unknown