Theranostic Nanoplatforms of Thiolated Reduced Graphene Oxide Nanosheets and Gold Nanoparticles

Applied Sciences

Angelo Nicosia¹  Placido Giuseppe Mineo¹  Giuseppe Consiglio¹  Giuseppe Forte²  Vanessa Sanfilippo³  Carmela Bonaccorso³  Lorena Maria Cucci³  Cristina Satriano³  Pascal Tomasella³ 

[1] Department of Chemical Sciences, University of Catania, viale Andrea Doria 6, 95125 Catania, Italy;Department of Pharmaceutical Sciences, University of Catania, viale Andrea Doria 6, 95125 Catania, Italy;Laboratory of Hybrid NanoBioInterfaces (NHBIL), Department of Chemical Sciences, University of Catania, viale Andrea Doria 6, 95125 Catania, Italy;

关键词: plasmonics;    nanorods;    nanospheres;    graphene toxicity;    atomic force microscopy;    thermogravimetry;   

DOI  :  10.3390/app10165529

来源: DOAJ

【 摘 要 】

In this study, graphene oxide (GO) and reduced-thiolated GO (rGOSH) were used as 2D substrate to fabricate nanocomposites with nanoparticles of gold nanospheres (AuNS) or nanorods (AuNR), via in situ reduction of the metal salt precursor and seed-mediated growth processes. The plasmonic sensing capability of the gold-decorated nanosheets were scrutinized by UV-visible (UV-VIS) spectroscopy. Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), thermogravimetric analyses (TGA), and atomic force microscopy (AFM) were performed in order to prove the actual reduction that occurred concomitantly with the thiolation of GO, the increase in the hydrophobic character as well as the size, and preferential gathering of the gold nanoparticles onto the nanosheet substrates, respectively. Moreover, the theoretical electronic and infrared absorption (UV-VIS and IR) spectra were calculated within a time-dependent approach of density functional theory (DFT). Eventually, in vitro cellular experiments on human neuroblastoma cells (SH-SY5Y line) were carried out in order to evaluate the nanotoxicity of the nanocomposites by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tetrazolium reduction (MTT) colorimetric assay. Results pointed out the promising potential of these hybrids as plasmonic theranostic platforms with different hydrophilic or hydrophobic features as well as cytotoxic effects against cancer cells.

【 授权许可】

Unknown