Biosynthetic Pathway and Metabolic Engineering of Succinic Acid

Frontiers in Bioengineering and Biotechnology

Xinjun Feng¹  Chuanle Fan²  Shengjie Sun³  Xiutao Liu³  Peng Xiong³  Guang Zhao⁴ 

[1] CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China;School of Chemical Engineering, University of Chinese Academy of Sciences, Beijing, China;School of Life Sciences and Medicine, Shandong University of Technology, Zibo, China;State Key Lab of Microbial Technology, Shandong University, Qingdao, China;

关键词: succinic acid;    biosynthesis pathways;    metabolic engineering;    CO2 fixation;    NADH/NAD + ratio;   

DOI  :  10.3389/fbioe.2022.843887

来源: DOAJ

【 摘 要 】

Succinic acid, a dicarboxylic acid produced as an intermediate of the tricarboxylic acid (TCA) cycle, is one of the most important platform chemicals for the production of various high value-added derivatives. As traditional chemical synthesis processes suffer from nonrenewable resources and environment pollution, succinic acid biosynthesis has drawn increasing attention as a viable, more environmentally friendly alternative. To date, several metabolic engineering approaches have been utilized for constructing and optimizing succinic acid cell factories. In this review, different succinic acid biosynthesis pathways are summarized, with a focus on the key enzymes and metabolic engineering approaches, which mainly include redirecting carbon flux, balancing NADH/NAD+ ratios, and optimizing CO2 supplementation. Finally, future perspectives on the microbial production of succinic acid are discussed.

【 授权许可】

Unknown