Calcium Carbonate Addition Improves L-Methionine Biosynthesis by Metabolically Engineered Escherichia coli W3110-BL

Frontiers in Bioengineering and Biotechnology

Bin Zhou³  Wang-Jie Wu³  Yue-Ying Xu³  Kun Niu³  Hai-Yan Zhou³  Yu-Guo Zheng³  Zhi-Qiang Liu³ 

[1]Engineering Research Center of Bioconversion and Biopurification, Ministry of Education, Zhejiang University of Technology, Hangzhou, China

[2]Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China

[3]The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, China

关键词: L-methionine;    metabolic flux;    Escherichia coli;    calcium carbonate;    flux balance analysis;   

DOI  :  10.3389/fbioe.2020.00300

来源: DOAJ

【 摘 要 】

L-Methionine (L-Met) is a sulfur-containing amino acid, which is one of the eight essential amino acids to human body. In this work, the fermentative production of L-Met with genetically engineered Escherichia coli W3110-BL in a 5-L fermentor was enhanced through supplement of Ca2+ into the fermentation medium. With the addition of 30 g/L calcium carbonate (CaCO3), the titer of L-Met and yield against glucose reached 1.48 g/L and 0.09 mol/mol glucose, 57.45% higher than those of the control, respectively. The flux balance analysis (FBA) revealed that addition of CaCO3 strengthened the tricarboxylic acid cycle and increased the intracellular ATP concentration by 39.28%. The re-distribution of carbon, ATP, and cofactors flux may collaborate to improve L-Met biosynthesis with E. coli W3110-BL. The regulation of citrate synthase and oxidative phosphorylation pathway was proposed to be important for overproduction of L-Met. These foundations provide helpful reference in the following metabolic modification or fermentation control for further improvement of L-Met biosynthesis.

【 授权许可】

Unknown