Long non-coding RNA ZFY-AS1 represses periodontitis tissue inflammation and oxidative damage via modulating microRNA-129-5p/DEAD-Box helicase 3 X-linked axis

Bioengineered

ZhiYuan Feng¹  Qingmei Liu²  Lin Cheng²  Jun Wang²  YuLing Fan³  Jue Cheng⁴ 

[1] Department of Orthodontics, Shanxi Provincial People’s Hospital, Taiyuan City, Shanxi Province, China;Department of Stomatology, Bethune Hospital, (Shanxi Academy of Medical Sciences), Taiyuan City, Shanxi Province, China;Department of Stomatology, School of Stomatology, Shanxi Medical University, Taiyuan City, Shanxi Province, China;Department of Stomatology, The Community Health Service Center of Beijing Jiao Tong University, Beijing City, China;

关键词: Long non-coding RNA ZFY-AS1;    microRNA-129-5p;    periodontitis;    oxidative damage;   

DOI  :  10.1080/21655979.2021.2019876

来源: DOAJ

【 摘 要 】

A large number of studies have manifested long non-coding RNA (lncRNA) is involved in the modulation of the development of periodontitis, but the specific mechanism has not been fully elucidated. The purpose of this study was to explore the biological function and latent molecular mechanism of lncZFY-AS1 in periodontitis. The results clarified lncZFY-AS1 and DEAD-Box Helicase 3 X-Linked (DDX3X) were up-regulated, but microRNA (miR)-129-5p was down-regulated in periodontitis. Knockdown of lncZFY-AS2 or overexpression of miR-129-5p decreased macrophage infiltration and periodontal membrane cell apoptosis, increased cell viability, repressed inflammatory factors and nuclear factor kappa B activation, reduced oxidative stress, but promoted nuclear factor-E2-related factor 2/heme oxygenase 1 expression. LncZFY-AS1 elevation further aggravated periodontitis inflammation, oxidative stress, and apoptosis. LncZFY competitively adsorbed miR-129-5p to mediate DDX3X expression. Knockdown lncZFY’s improvement effect on periodontitis was reversed by depressive miR-129-5p or enhancive DDX3X. In conclusion, these data suggest lncZFY-AS1 promotes inflammatory injury and oxidative stress in periodontitis by competitively binding to miR-129-5p and mediating DDX3X expression. LncZFY-AS1/miR-129-5p/DDX3X may serve as a novel molecular target for treatment of periodontitis in the future.

【 授权许可】

Unknown