A Cell-Adhesive Plasma Polymerized Allylamine Coating Reduces the In Vivo Inflammatory Response Induced by Ti6Al4V Modified with Plasma Immersion Ion Implantation of Copper

Journal of Functional Biomaterials

J. Barbara Nebe¹  Andreas Podbielski²  Rainer Bader³  Carmen Zietz³  Andreas Hoene⁴  Maciej Patrzyk⁴  Gerold Lukowski⁵  Birgit Finke⁶  Martin Polak⁶  Michael Schlosser⁷  Uwe Walschus⁷  Silke Lucke⁷ 

[1] Department of Cell Biology, University Medical Center Rostock, 18057 Rostock, Germany;Department of Medical Microbiology, Virology and Hospital Hygiene, University Medical Center Rostock, 18057 Rostock, Germany;Department of Orthopaedics, University Medical Center Rostock, 18057 Rostock, Germany;Department of Surgery, University Medical Center Greifswald, 17475 Greifswald, Germany;Institute of Marine Biotechnology, 17489 Greifswald, Germany;Leibniz Institute for Plasma Science and Technology (INP), 17489 Greifswald, Germany;Research Group of Predictive Diagnostics, Department of Medical Biochemistry and Molecular Biology, University Medical Center Greifswald, 17475 Greifswald, Germany;

关键词: titanium;    copper;    antibacterial properties;    plasma polymerization;    plasma immersion ion implantation;    biocompatibility;    morphometry;    inflammatory response;   

DOI  :  10.3390/jfb8030030

来源: DOAJ

【 摘 要 】

Copper (Cu) could be suitable to create anti-infective implants based on Titanium (Ti), for example by incorporating Cu into the implant surface using plasma immersion ion implantation (Cu-PIII). The cytotoxicity of Cu might be circumvented by an additional cell-adhesive plasma polymerized allylamine film (PPAAm). Thus, this study aimed to examine in vivo local inflammatory reactions for Ti6Al4V implants treated with Cu-PIII (Ti-Cu), alone or with an additional PPAAm film (Ti-Cu-PPAAm), compared to untreated implants (Ti). Successful Cu-PIII and PPAAm treatment was confirmed with X-ray Photoelectron Spectroscopy. Storage of Ti-Cu and Ti-Cu-PPAAm samples in double-distilled water for five days revealed a reduction of Cu release by PPAAm. Subsequently, Ti, Ti-Cu and Ti-Cu-PPAAm samples were simultaneously implanted into the neck musculature of 24 rats. After 7, 14 and 56 days, peri-implant tissue was retrieved from 8 rats/day for morphometric immunohistochemistry of different inflammatory cells. On day 56, Ti-Cu induced significantly stronger reactions compared to Ti (tissue macrophages, antigen-presenting cells, T lymphocytes) and to Ti-Cu-PPAAm (tissue macrophages, T lymphocytes, mast cells). The response for Ti-Cu-PPAAm was comparable with Ti. In conclusion, PPAAm reduced the inflammatory reactions caused by Cu-PIII. Combining both plasma processes could be useful to create antibacterial and tissue compatible Ti-based implants.

【 授权许可】

Unknown