Effect of Long-Term Continuous Light Exposure and Western Diet on Adropin Expression, Lipid Metabolism, and Energy Homeostasis in Rats

Biology

AbdullahIbrahim Farraj¹  Manal Abudawood¹  GhadeerS. Aljuraiban²  MahmoudMustafa Ali Abulmeaty²  KhalidS. Alnumair²  Suhail Razak²  AliMadi Almajwal²  Amal Fawzy³  MaiMohammed Hasan⁴ 

[1] Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11362, Saudi Arabia;Department of Community Health Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 11362, Saudi Arabia;Department of Medical Biochemistry, School of Medicine, Zagazig University, Zagazig 44519, Egypt;Department of Medical Physiology, School of Medicine, Zagazig University, Zagazig 44519, Egypt;

关键词: Adropin;    continuous light;    Western diet;    RORα;    Rev-erb-α;    energy expenditure;   

DOI  :  10.3390/biology10050413

来源: DOAJ

【 摘 要 】

Long-term continuous light exposure (CL) and western diet (WD) effects on Adropin expression, RORα, and Rev-erb-α nuclear receptors and energy homeostasis were studied in rats. Thirty-two male Wistar rats (250–290 g) were enrolled for 3 months in the following groups (n = 8/group): (a) Normal control group (NC), (b) CL group, (c) WD group, and (d) CL + WD group. Then, indirect calorimetry and food intake (FI) were measured. Finally, Adropin, hormone-sensitive lipase (HSL), adipocyte triglyceride lipase (ATGL), and free fatty acids (FFA) were measured. Additionally, the histopathology and gene expression of Enho, RORα, and Rev-erb-α genes were done. The CL alone elevated the Adropin plasma level and gene expression, increased RORα expression, and decreased the Rev-erb-α nuclear receptor expression mainly in the liver and kidney. Besides, CL increased the total energy expenditure (TEE) and decreased the respiratory quotient. WD alone or in combination with the CL reversed gene expression of Enho, RORα, and Rev-erb-α. Combined CL and WD increased the TEE, reduced the food intake, increased the ATGL, and reduced the Adropin level in addition to widespread degenerative changes in the liver, spleen, and renal tissues. The deleterious effects of CL and WD on energy homeostasis may include Adropin with the involvement of the RORα and Rev-erb-α nuclear receptors.

【 授权许可】

Unknown