Advances in Diagnostic Approaches for Viral Etiologies of Diarrhea: From the Lab to the Field

Frontiers in Microbiology

Durlav Prasad Bora¹  Ahmed S. Abdel-Moneim³  Krisztián Bányai⁴  Anastasia N. Vlasova⁵  Nobumichi Kobayashi⁶  Raj Kumar Singh⁷  Kumaragurubaran Karthik⁸  Maged Gomaa Hemida^1,10  Kuldeep Dhama^1,11  Ruchi Tiwari^1,12  Atul Kumar Verma^1,13  Yashpal Singh Malik^1,13  Souvik Ghosh^1,14  Naveen Kumar^1,15  Nadia Touil^1,16 

[1] Immunology, College of Veterinary Sciences, DUVASU, Mathura, India;0Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt;1Department of Microbiology, College of Medicine, Taif University, Taif, Saudi Arabia;2Department of Virology, Faculty of Veterinary Medicine, Beni Suef University, Beni Suef, Egypt;3Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary;4Food Animal Health Research Program, Department of Veterinary Preventive Medicine, CFAES, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH, United States;5School of Medicine, Sapporo Medical University, Sapporo, Japan;Central University Laboratory, Tamil Nadu Veterinary and Animal Sciences University, Chennai, India;Department of Biomedical Sciences, One Health Center for Zoonoses and Tropical Veterinary Medicine, Ross University School of Veterinary Medicine, Basseterre, Saint Kitts and Nevis;Department of Microbiology and Parasitology, College of Veterinary Medicine, King Faisal University, Al-Hufuf, Saudi Arabia;Department of Microbiology, College of Veterinary Science, Assam Agricultural University, Guwahati, India;;Department of Veterinary Microbiology &Division of Biological Standardization, Indian Council of Agricultural Research-Indian Veterinary Research Institute, Izatnagar, India;Division of Pathology, Indian Council of Agricultural Research-Indian Veterinary Research Institute, Izatnagar, India;ICAR-National Institute of High Security Animal Diseases, OIE Reference Laboratory for Avian Influenza, Bhopal, India;Laboratoire de Biosécurité et de Recherche, Hôpital Militaire d’Instruction Mohammed V, Rabat, Morocco;

关键词: enteric virus;    infection;    diagnosis;    cell culture;    molecular tests;   

DOI  :  10.3389/fmicb.2019.01957

来源: DOAJ

【 摘 要 】

The applications of correct diagnostic approaches play a decisive role in timely containment of infectious diseases spread and mitigation of public health risks. Nevertheless, there is a need to update the diagnostics regularly to capture the new, emergent, and highly divergent viruses. Acute gastroenteritis of viral origin has been identified as a significant cause of mortality across the globe, with the more serious consequences seen at the extremes of age groups (young and elderly) and immune-compromised individuals. Therefore, significant advancements and efforts have been put in the development of enteric virus diagnostics to meet the WHO ASSURED criteria as a benchmark over the years. The Enzyme-Linked Immunosorbent (ELISA) and Polymerase Chain Reaction (PCR) are the basic assays that provided the platform for development of several efficient diagnostics such as real-time RT-PCR, loop-mediated isothermal amplification (LAMP), polymerase spiral reaction (PSR), biosensors, microarrays and next generation sequencing. Herein, we describe and discuss the applications of these advanced technologies in context to enteric virus detection by delineating their features, advantages and limitations.

【 授权许可】

Unknown