The Anti-Angiogenic Activity of a Cystatin F Homologue from the Buccal Glands of Lampetra morii

Marine Drugs

Rong Xiao¹  Jihong Wang¹  Mingru Zhu¹  Bowen Li¹ 

[1] School of Life Sciences, Liaoning Normal University, Dalian 116081, China;

关键词: Lampetra morii;    buccal gland;    cystatin F;    anti-angiogenesis;    cystatin superfamily;   

DOI  :  10.3390/md16120477

来源: DOAJ

【 摘 要 】

Cystatins are a family of cysteine protease inhibitors which are associated with a variety of physiological and pathological processes in vivo. In the present study, the cDNA sequence of a cystatin F homologue called Lm-cystatin F was cloned from the buccal glands of Lampetra morii. Although Lm-cystatin F shares a lower homology with cystatin superfamily members, it is also composed of a signal peptide and three highly conserved motifs, including the G in the N-terminal, QXVXG, as well as the PW in the C-terminal of the sequence. After sequence optimization and recombination, the recombinant protein was expressed as a soluble protein in Escherichia coli with a molecular weight of 19.85 kDa. Through affinity chromatography and mass spectrometry analysis, the purified protein was identified as a recombinant Lm-cystatin F (rLm-cystatin F). Additionally, rLm-cystatin F could inhibit the activity of papain. Based on MTT assay, rLm-cystatin F inhibited the proliferation of human umbilical vein endothelial cells (HUVECs) dose dependently with an IC₅₀ of 5 μM. In vitro studies show that rLm-cystatin F suppressed the adhesion, migration, invasion, and tube formation of HUVECs, suggesting that rLm-cystatin F possesses anti-angiogenic activity, which provides information on the feeding mechanisms of Lampetra morii and insights into the application of rLm-cystatin F as a potential drug in the future.

【 授权许可】

Unknown