Acaricidal Efficacy of Plants from Ecuador, Ambrosia peruviana (Asteraceae) and Lepechinia mutica (Lamiaceae) against Larvae and Engorged Adult Females of the Common Cattle Tick, Rhipicephalus microplus

Veterinary Sciences

Lucía Guzmán¹  Jorge Ramírez²  James Calva²  Gianluca Gilardoni²  Eduardo Valarezo²  Daniel Hidalgo²  Catalina Rey-Valeirón³  Jorge Luis Malla⁴ 

[1] Departamento de Ciencias Biológicas y Agropecuarias, Facultad de Ciencias Exactas y Naturales, Universidad Técnica Particular de Loja, San Cayetano Alto s/n, Loja 110107, Ecuador;Departamento de Química, Facultad de Ciencias Exactas y Naturales, Universidad Técnica Particular de Loja, San Cayetano Alto s/n, Loja 110107, Ecuador;Laboratorio de Investigación en Parasitología Veterinaria, Intercomunal Coro-La Vela, Universidad Nacional Experimental Francisco de Miranda, Coro 4101, Venezuela;Titulación en Ingeniería Agropecuaria, Facultad de Ciencias Biológicas y Agropecuarias, Universidad Técnica Particular de Loja, Titulación en Ingeniería Agropecuaria, San Cayetano Alto s/n, Loja 110107, Ecuador;

关键词: cattle tick;    Rhipicephalus microplus;    control;    efficacy;    essential oils;    Ambrosia peruviana;   

DOI  :  10.3390/vetsci9010023

来源: DOAJ

【 摘 要 】

Control measures against common cattle tick Rhipicephalus microplus are of the upmost importance because of considerable, deleterious impact on a farm’s economy. Due to resistance phenomena to synthetic acaricides being a constraint in affected farms, the search for plant derivatives as acaricides has increased dramatically in recent years. In this work, essential oils obtained from two Ecuadorian plants, Ambrosia peruviana and Lepechinia mutica (EO_Ap, EO_Lm), traditionally used as insecticides in indigenous communities, were studied on larvae and engorged females at the parasitic stages of R. microplus. Larvae and females were treated with five (0.0625, 0.125, 0.25, 0.50 and 1%) and six concentrations (0.125, 0.25, 0.50, 1, 2 and 4%), respectively, of each EOs_Ap/Lm. A 98–99% larval mortality was achieved with 0.5% of both EOs_Ap/Lm. EO_Ap inhibited oviposition and egg hatching up to 82% and 80%, respectively, and had an overall efficacy of 93.12%. Efficacy of EO_Lm was 72.84%, due to the low influence of EO_Lm on reproductive parameters. By steam distillation and GC-MS analysis, γ-Curcumene was identified as the main constituent (52.02%) in the EO_Ap and Shyobunol (10.80%) in EO_Lm. The results suggest that major components of both essential oils should be further studied as promissory acaricides against R. microplus.

【 授权许可】

Unknown