How can fertilization regimes and durations shape earthworm gut microbiota in a long-term field experiment?

Ecotoxicology and Environmental Safety

Dong Zhu¹  Bing-Jie Jin²  Andreas Richter³  Yong-Guan Zhu⁴  Xiao-Ru Yang⁵  Patrick O'Connor⁶  Xian-Yong Lin⁷  Qing-Fang Bi⁸  Yu-Gen Jiang⁸  Bang-Xiao Zheng⁹ 

[1] Division of Terrestrial Ecosystem Research, Department of Microbiology and Ecosystem Science, Centre for Microbiology and Environmental Systems Science, University of Vienna, Vienna, Austria;Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China;Centre for Global Food and Resources, University of Adelaide, Adelaide 5005, Australia;Division of Terrestrial Ecosystem Research, Department of Microbiology and Ecosystem Science, Centre for Microbiology and Environmental Systems Science, University of Vienna, Vienna, Austria;Faculty of Biological and Environmental Sciences, Ecosystems and Environment Research Programme, University of Helsinki, Lahti 15140, Finland;Fuyang Agricultural Technology Popularization Center, Hangzhou 311400, PR China;Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China;MOE Key Laboratory of Environment Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, PR China;State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China;

关键词: Organic-inorganic fertilization;    Earthworm species;    Gut microbiota;    Functional genes;    16S rRNA;   

DOI  :  

来源: DOAJ

【 摘 要 】

The positive roles of earthworms on soil functionality has been extensively documented. The capacity of the earthworm gut microbiota on decomposition and nutrient cycling under long-term fertilization in field conditions has rarely been studied. Here, we report the structural, taxonomic, and functional responses of Eisenia foetida and Pheretima guillelmi gut microbiota to different fertilization regimes and durations using 16S rRNA gene-based Illumina sequencing and high-throughput quantitative PCR techniques. Our results revealed that the core gut microbiota, especially the fermentative bacteria were mainly sourced from the soil, but strongly stimulated with species-specificity, potential benefits for the host and soil health. The functional compositions of gut microbiota were altered by fertilization with fertilization duration being more influential than fertilization regimes. Moreover, the combination of organic and inorganic fertilization with the longer duration resulted in a higher richness and connectivity in the gut microbiota, and also their functional potential related to carbon (C), nitrogen, and phosphorus cycling, particularly the labile C decomposition, denitrification, and phosphate mobilization. We also found that long-term inorganic fertilization increased the abundance of pathogenic bacteria in the P. guillelmi gut. This study demonstrates that understanding earthworm gut microbiota can provide insights into how agricultural practices can potentially alter soil ecosystem functions through the interactions between soil and earthworm gut microbiotas.

【 授权许可】

Unknown