Monitoring of oil hydrocarbons pollution in the Sea of Japan, based on detection of marker genes in microbial communities

3rd International Conference Environment and Sustainable Development of Territories: Ecological Challenges of the 21st Century

生态环境科学;地球科学;经济学

Kim, A.V.^1 ; Buzoleva, L.S.^1,2 ; Bogatyrenko, E.A.^1 ; Zemskaya, T.I.^3 ; Mamaeva, E.V.^3

Far Eastern Federal University, Sukhanova Str. 8, Vladivostok

690091, Russia^1

Somov Research Institute of Epidemiology and Microbiology, Sel'skaya str, 1, Vladivostok

690087, Russia^2

Limnological Institute, Siberian Branch, Russian Academy of Sciences, Ulan-Batorskaya str, 3, Irkutsk

664033, Russia^3

关键词: Anthropogenic pollution;    Based on detections;    Functional genes;    Inshore waters;    Microbial communities;    Molecular genetics;    Oil hydrocarbons;    Sea of Japan;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/107/1/012061/pdf
DOI  :  10.1088/1755-1315/107/1/012061

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

By means of molecular biology techniques, metabolic potential of microbial communities within the regions of inshore water areas in the Sea of Japan with various anthropogenic load was explored. Presence of functional genes, responsible for oil hydrocarbons destruction, for microbial communities within the regions of inshore water areas in the Sea of Japan was first researched. In total microbial DNA from water mass in the regions with chronic anthropogenic pollution, the genes, responsible for oxidation of broad range of n-alkanes and polycyclic aromatic hydrocarbons, were found. Detection of marker genes in the background water area (in the Vostok Bay) was ever indicating ecological deterioration within this territory. Thereby, it was demonstrated, that molecular genetic methods, aimed at marker gene detection in total bacterial DNA from environment objects, proved themselves to be more effective technique for identification of oil hydrocarbons water pollution, in comparison with trivial culturable methods.