Mycoremediation of Soils Polluted with Trichloroethylene: First Evidence of Pleurotus Genus Effectiveness

Applied Sciences

Begoña Mayans¹  Raquel Camacho-Arévalo¹  Rafael Antón-Herrero¹  Enrique Eymar¹  Carlos García-Delgado²  Consuelo Escolástico³  Norbert Nägele⁴  Cynthia Alcántara⁴ 

[1] Department of Agricultural Chemistry and Food Sciences, Autonomous University of Madrid, 28049 Madrid, Spain;Department of Geology and Geochemistry, Autonomous University of Madrid, 28049 Madrid, Spain;Department of Organic and Bio-organic Chemistry, Faculty of Sciences, Universidad Nacional de Educación a Distancia, 28040 Madrid, Spain;Kepler Ingeniería y Ecogestión, S.L, 09001 Burgos, Spain;

关键词: trichloroethylene;    mycoremediation;    Pleurotus;    bioremediation;    biodegradation;    pollution;   

DOI  :  10.3390/app11041354

来源: DOAJ

【 摘 要 】

Trichloroethylene (TCE) is a proven carcinogenic chlorinated organic compound widely used as a solvent in industrial cleaning solutions; it is easily found in the soil, air, and water and is a hazardous environmental pollutant. Most studies have attempted to remove TCE from air and water using different anaerobic bacteria species. In addition, a few have used white-rot fungi, although there are hardly any in soil. The objective of the present work is to assess TCE removal efficiency using two species of the genus Pleurotus that have not been tested before: Pleurotus ostreatus and Pleurotus eryngii, growing on a sandy loam soil. These fungi presented different intra- and extracellular enzymatic systems (chytochrome P450 (CYP450), laccase, Mn peroxidase (MnP)) capable of aerobically degrading TCE to less harmful compounds. The potential toxicity of TCE to P. ostreatus and P. eryngii was firstly tested in a TCE-spiked liquid broth (70 mg L⁻¹ and 140 mg L⁻¹) for 14 days. Then, both fungi were assessed for their ability to degrade the pollutant in sandy loam soil spiked with 140 mg kg⁻¹ of TCE. P. ostreatus and P. eryngii improved the natural dissipation of TCE from soil by 44%. Extracellular enzymes were poorly expressed, but mainly in the presence of the contaminant, in accordance with the hypothesis of the involvement of CYP450.

【 授权许可】

Unknown