Transcriptome Analysis Reveals Genes Involved in Thermogenesis in Two Cold-Exposed Sheep Breeds

Genes

Allan Degen¹  HuitongT. Zhou²  JonG. H. Hickford²  YunshengS. Zhang³  Xiukun Wu⁴  KaixiX. Ji⁵  Dan Jiao⁵  Guo Yang⁵  JianweiW. Zhou⁶  Hu Liu⁶  WenqiangQ. Wang⁶ 

[1] Desert Animal Adaptations and Husbandry, Wyler Department of Dryland Agriculture, Blaustein Institutes for Desert Research, Ben-Gurion University of Negev, Beer Sheva l8410500, Israel;Gene-Marker Laboratory, Department of Agricultural Sciences, Lincoln University, Lincoln 7647, New Zealand;Institute of Animal Husbandry, Xinjiang Academy of Animal Science, Xinjiang 830000, China;Key Laboratory of Desert and Desertification, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China;Northwest Institute of Ecological Environment and Resources, Chinese Academy of Sciences, Lanzhou 730070, China;School of Life Sciences, Lanzhou University, Lanzhou 730070, China;

关键词: sheep;    cold exposure;    transcriptome;    adipose tissue;   

DOI  :  10.3390/genes12030375

来源: DOAJ

【 摘 要 】

Thermogenesis plays an important role in the survival of sheep exposed to low temperatures; however, little is known about the genetic mechanisms underlying cold adaptation in sheep. We examined 6 Altay (A) and 6 Hu (H) six-month-old ewe lambs. Altay sheep are raised in northern China and are adapted to dry, cold climates, while Hu sheep are raised in southern China and are adapted to warm, humid climates. Each breed was divided into two groups: chronic cold sheep, exposed to −5 °C for 25 days (3 A^c; 3 H^c), and thermo-neutral sheep, maintained at 20 °C (3 A^w; 3 H^w). The transcriptome profiles of hypothalamus, tail-fat and perirenal fat tissues from these four groups were determined using paired-end sequencing for RNA expression analysis. There are differences in cold tolerance between Hu and Altay sheep. Under cold exposure of the lambs: (1) UCP1-dependent thermogenesis and calcium- and cAMP-signaling pathways were activated; and (2) different fat tissues were activated in Hu and Altay lambs. Several candidate genes involved in thermogenesis including UCP1, ADRB3, ADORA2A, ATP2A1, RYR1 and IP6K1 were identified. Molecular mechanisms of thermogenesis in the sheep are discussed and new avenues for research are suggested.

【 授权许可】

Unknown