Protein purification and crystallization of HLA-A∗02:01 in complex with SARS-CoV-2 peptides

STAR Protocols

Dhilshan Jayasinghe¹  Christopher Szeto²  Demetra S.M. Chatzileontiadou³  Stephanie Gras³ 

[1] Corresponding author;Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800, Australia;Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, VIC 3083, Australia;

关键词: Immunology;    Protein expression and purification;    X-ray Crystallography;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Understanding T-cell responses requires identifying viral peptides presented by human leukocyte antigens (HLAs). X-ray crystallography can be used to visualize their presentation. This protocol describes the expression, purification, and crystallization of HLA-A∗02:01, one of the most frequent HLA in the global population in complex with peptides derived from the SARS-CoV-2 nucleocapsid protein. This protocol can be applied to different HLA class I molecules bound to other peptides.For complete details on the use and execution of this protocol, please refer to Szeto et al. (2021).

【 授权许可】

Unknown