Characterization and Proteomic Analysis of Novel Rice Lesion Mimic Mutant with Enhanced Disease Resistance

Rice Science

Chen Jianping¹  Liang Weifang²  Yan Chengqi³  Xu Zhengjin⁴  Lin Qiujun⁴  Fu Yuwen⁴  Wu Yuanhua⁴  Mei Qiong⁴  Yu Chulang⁵  Zhou Jie⁶  Wang Xuming⁶  Yang Yong⁶  Chen Xinyu⁷ 

[1] Corresponding author.;College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China;Institute of Biotechnology, Ningbo Academy of Agricultural Science, Ningbo 315040, China;Plant Pathogen Laboratory, College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China;State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products/Key Laboratory of Biotechnology for Plant Protection, Ministry of Agriculture and Rural Affairs of China/Zhejiang Provincial Key Laboratory of Biotechnology for Plant Protection, Institute of Plant Virology, Ningbo University, Ningbo 315211, China;State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products/Key Laboratory of Biotechnology for Plant Protection, Ministry of Agriculture and Rural Affairs of China/Zhejiang Provincial Key Laboratory of Biotechnology for Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China;State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forest University, Hangzhou 311300, China;

关键词: lesion mimic mutant;    H2O2;    disease resistance;    pathogenesis-related protein;    resistance- related pathway;   

DOI  :  

来源: DOAJ

【 摘 要 】

Lesion mimic mutants (LMMs) are plants that spontaneously form lesions without pathogen infection or external stimulus and exhibit resistance to pathogens. Here, a rice LMM was created by ethyl methane sulfonate mutagenesis, named as hpil (hydrogen peroxide induced lesion). Diaminobenzidine and trypan blue staining showed that large amounts of H2O2 were produced and cell death was occurred at and around the parts of lesion mimic in the rice leaves. The phenotype of hpil is controlled by a single recessive gene, localized at a 2 Mb interval on chromosome 2. The data suggested that hpil is a novel LMM with enhanced bacterial and fungal disease resistance, and multiple pathogenesis-related proteins (PRs) were up-regulated. The proteomes of leaves at three positions (different degrees of lesion mimic severity) were characterized in hpil compared with its wild type plant. Differentially expressed proteins were detected by two dimensional difference gel electrophoresis and 274 proteins were identified by MALDI TOF/TOF™. These proteins were related to metabolic process, cellular process and response to stimulus, with mostly down-regulated in hpil leaves. Many of these proteins were related to the Calvin cycle, photosynthetic electron transport chain, glycolysis/gluconeogenesis and phosphonates pathways. Some resistance-related proteins including 14-3-3 proteins, OsPR10 and antioxidases such as peroxidase, superoxide dismutase and ascorbate peroxidase were up-regulated in leaves with lesion mimic. These results provide the foundation for cloning of the target gene and shed light on the mechanism involved in autoimmunity of rice.

【 授权许可】

Unknown