Efficacy of Chondroprotective Food Supplements Based on Collagen Hydrolysate and Compounds Isolated from Marine Organisms

Marine Drugs

Helen Louton¹  Roland Schauer²  Tibor Kožár³  John W. Hudson⁴  Anna Kozarova⁴  Monika Burg-Roderfeld⁵  Mahena Jährling-Butkus⁵  Jürgen Steinmeyer⁶  Athanasios K. Petridis⁷  Peter Engelhard⁸  Ning Zhang⁸  Thomas Eckert⁸  Ruiyan Zhang⁸  Hans-Christian Siebert⁸  Karsten Hesse⁹ 

[1] Animal Health and Animal Welfare, Faculty of Agricultural and Environmental Sciences, University of Rostock, Justus-von-Liebig-Weg 6b, 18059 Rostock, Germany;Biochemisches Institut, Christian-Albrechts Universität Kiel, Olshausenstrasse 40, 24098 Kiel, Germany;Center for Interdisciplinary Biosciences, Technology and Innovation Park, P. J. Šafárik University, Jesenná 5, 04001 Košice, Slovakia;Department of Biomedical Sciences, University of Windsor, Windsor, ON N9B 3P4, Canada;Institut für Veterinärphysiolgie und Biochemie, Fachbereich Veterinärmedizin, Justus-Liebig-Universität Gießen, Frankfurter Str. 100, 35392 Gießen, Germany;Laboratory for Experimental Orthopaedics, Department of Orthopaedics, Justus Liebig University Giessen, Paul-Meimberg-Str. 3, 35392 Giessen, Germany;Medical School, Heinrich-Heine-Universität Düsseldorf, Universitätsstr. 1, 40225 Düsseldorf, Germany;RI-B-NT—Research Institute of Bioinformatics and Nanotechnology, Schauenburgerstr. 116, 24118 Kiel, Germany;Tierarztpraxis Dr. Karsten Hesse, Rathausstraße 16, 35460 Staufenberg, Germany;

关键词: osteoarthritis;    collagen hydrolysate;    sulfated N-acetyl glucosamine;    sialic acids;    eicosapentaenoic acid (EPA);    MMP-3;   

DOI  :  10.3390/md19100542

来源: DOAJ

【 摘 要 】

Osteoarthritis belongs to the most common joint diseases in humans and animals and shows increased incidence in older patients. The bioactivities of collagen hydrolysates, sulfated glucosamine and a special fatty acid enriched dog-food were tested in a dog patient study of 52 dogs as potential therapeutic treatment options in early osteoarthritis. Biophysical, biochemical, cell biological and molecular modeling methods support that these well-defined substances may act as effective nutraceuticals. Importantly, the applied collagen hydrolysates as well as sulfated glucosamine residues from marine organisms were strongly supported by both an animal model and molecular modeling of intermolecular interactions. Molecular modeling of predicted interaction dynamics was evaluated for the receptor proteins MMP-3 and ADAMTS-5. These proteins play a prominent role in the maintenance of cartilage health as well as innate and adapted immunity. Nutraceutical data were generated in a veterinary clinical study focusing on mobility and agility. Specifically, key clinical parameter (MMP-3 and TIMP-1) were obtained from blood probes of German shepherd dogs with early osteoarthritis symptoms fed with collagen hydrolysates. Collagen hydrolysate, a chondroprotective food supplement was examined by high resolution NMR experiments. Molecular modeling simulations were used to further characterize the interaction potency of collagen fragments and glucosamines with protein receptor structures. Potential beneficial effects of collagen hydrolysates, sulfated glycans (i.e., sulfated glucosamine from crabs and mussels) and lipids, especially, eicosapentaenoic acid (extracted from fish oil) on biochemical and physiological processes are discussed here in the context of human and veterinary medicine.

【 授权许可】

Unknown