First Report of Agrobacterium tumefaciens mediated genetic transformation of aquatic Rice paddy herb (Limnophila aromatica)

Turkish Journal of Agriculture: Food Science and Technology

Allah Bakhsh¹  Mehmet Karataş¹  Muhammad Abu Bakar Zia¹  Gökhan Sadı²  Muhammad Aasim²  Muhammet Doğan²  Khalid Mahmood Khawar³ 

[1] Department of Agricultural Genetic Engineering, Faculty of Agricultural Sciences and Technology, Niğde University, 51240 Niğde;Department of Biology, Kamil Özdağ Faculty of Sciences, Karamanoğlu Mehmetbey University, 70200 Karaman;Department of Field Crops, Faculty of Agriculture, Ankara University, 0611 Dışkapı/Ankara;

关键词: Agrobacterium;    Genetic transformation;    Shoot tip;    GUS;    nptII;   

DOI  :  10.24925/turjaf.v4i8.642-645.747

来源: DOAJ

【 摘 要 】

The study presents first report of Agrobacterium mediated genetic transformation in Rice paddy herb (Linmophila aromatica). A. tumefaciens strain C58C1 harboring pBin19 Plasmid containing β-glucuronidase (GUS)and neomycin phosphotransferase II (nptII) gene, under the control of 35S promoter and NOS terminator was used. Shoot tip explants were inoculated for 30 min followed by co-cultivation for 72 h and selected on agar semi solidified MS medium containing 100 mg/l Kanamycin and 1.0 mg/l BA; whereas total number of 78 putative transgenic shoots were obtained. The shoots were rooted on MS medium containing 1.0 mg/l IBA and 100 mg/l Kanamycin where 43 plants survived and rooted. Expression of GUS gene in the putative transgenics was confirmed by histochemical GUS assay. Visible localised gus expression was noted in a few cells and callus tissues of 4 plantlets that were photographed using compound light microscope.

【 授权许可】

Unknown