Bioactive Low Molecular Weight Keratin Hydrolysates for Improving Skin Wound Healing

Polymers

Cristina Tablet¹  Ioana-Rodica Stanculescu²  Carmen Gaidau³  Maria Stanca³  Brindusa Georgiana Dumitriu⁴  Luiza Mariana Tanase⁴  Manuela Diana Ene⁴  Laura Olariu⁴ 

[1] Department of Physical Chemistry, University of Bucharest, 4–12 Regina Elisabeta Bd., 030018 Bucharest, Romania;Horia Hulubei National Institute of Research and Development for Physics and Nuclear Engineering, 30 Reactorului Str., 077125 Magurele, Romania;Leather Research Department, National Institute for Textiles and Leather Division Leather and Footwear Research Institute (ICPI), 93 Ion Minulescu Street, 031215 Bucharest, Romania;SC Biotehnos SA, 3–5 Gorunului Street, 075100 Otopeni, Romania;

关键词: keratin hydrolysate;    bioactive keratin;    skin homeostasis restoration;    skin wound healing;   

DOI  :  10.3390/polym14061125

来源: DOAJ

【 摘 要 】

Keratin biomaterials with high molecular weights were intensively investigated but few are marketed due to complex methods of extraction and preparation and limited understanding of their influence on cells behavior. In this context the aim of this research was to elucidate decisive molecular factors for skin homeostasis restoration induced by two low molecular weight keratin hydrolysates extracted and conditioned through a simple and green method. Two keratin hydrolysates with molecular weights of 3758 and 12,400 Da were physico-chemically characterized and their structure was assessed by circular dichroism (CD) and FTIR spectroscopy in view of bioactive potential identification. Other investigations were focused on several molecular factors: α1, α2 and β1 integrin mediated signals, cell cycle progression in pro-inflammatory conditions (TNFα/LPS stimulated keratinocytes and fibroblasts) and ICAM-1/VCAM-1 inhibition in human vascular endothelial cells. Flow cytometry techniques demonstrated a distinctive pattern of efficacy: keratin hydrolysates over-expressed α1 and α2 subunits, responsible for tight bounds between fibroblasts and collagen or laminin 1; both actives stimulated the epidermal turn-over and inhibited VCAM over-expression in pro-inflammatory conditions associated with bacterial infections. Our results offer mechanistic insights in wound healing signaling factors modulated by the two low molecular weight keratin hydrolysates which still preserve bioactive secondary structure.

【 授权许可】

Unknown