λ-Carrageenan Suppresses Tomato Chlorotic Dwarf Viroid (TCDVd) Replication and Symptom Expression in Tomatoes

Marine Drugs

Alan T. Critchley¹  Rudra P. Singh²  Wajahatullah Khan³  Jatinder S. Sangha⁴  Saveetha Kandasamy⁴  Balakrishnan Prithiviraj⁴  Navratan Singh Bahia⁴ 

[1] Acadian Seaplants Limited, 30 Brown Avenue, Dartmouth, NS B3B 1X8, Canada;Agriculture and Agri-Food Canada, 850 Lincoln Rd., Fredericton, NB E3B 4Z7, Canada;Basic Sciences Department, King Saud Bin Abdul Aziz University for Health Sciences,P.O. Box 22490, Riyadh 11426, Saudi Arabia;Department of Environmental Sciences, Faculty of Agriculture, Dalhousie University, P.O. Box 550, Truro, NS B2N 5E3, Canada;

关键词: tomato chlorotic dwarf viroid (TCDVd);    carrageenans;    induced resistance;   

DOI  :  10.3390/md13052875

来源: DOAJ

【 摘 要 】

The effect of carrageenans on tomato chlorotic dwarf viroid (TCDVd) replication and symptom expression was studied. Three-week-old tomato plants were spray-treated with iota(ɩ)-, lambda(λ)-, and kappa(κ)-carrageenan at 1 g·L−1 and inoculated with TCDVd after 48 h. The λ-carrageenan significantly suppressed viroid symptom expression after eight weeks of inoculation, only 28% plants showed distinctive bunchy-top symptoms as compared to the 82% in the control group. Viroid concentration was reduced in the infected shoot cuttings incubated in λ-carrageenan amended growth medium. Proteome analysis revealed that 16 tomato proteins were differentially expressed in the λ-carrageenan treated plants. Jasmonic acid related genes, allene oxide synthase (AOS) and lipoxygenase (LOX), were up-regulated in λ-carrageenan treatment during viroid infection. Taken together, our results suggest that λ-carrageenan induced tomato defense against TCDVd, which was partly jasmonic acid (JA) dependent, and that it could be explored in plant protection against viroid infection.

【 授权许可】

Unknown