Genomic Characterization of Multidrug-Resistant Escherichia coli BH100 Sub-strains

Frontiers in Microbiology

Andrey A. Zamyatnin¹  Anne Pinto²  Edmar Chartone-Souza²  Vasco Azevedo²  Luis de Jesus²  Marcus Canario²  Aristoteles Goes-Neto²  Andréa M. A. Nascimento²  Henrique C. P. Figueiredo²  Flavia Aburjaile³  Preetam Ghosh⁴  Siomar Soares⁵  Debmalya Barh⁶  Rodrigo Carvalho⁷  Bertram Brenig⁸  Rommel Ramos⁹ 

[1] Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia;Departamento de Genética, Ecologia e Evolução, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil;Departamento de Genética, Universidade Federal de Pernambuco, Recife, Brazil;Department of Computer Science, Virginia Commonwealth University, Richmond, VA, United States;Departmento de Microbiologia, Imunologia e Parasitologia, Universidade Federal do Triangulo Mineiro, Uberaba, Brazil;Institute of Integrative Omics and Applied Biotechnology, Purba Medinipur, India;Institute of Molecular Medicine, Sechenov First Moscow State Medical University, Moscow, Russia;Institute of Veterinary Medicine, University of Göttingen, Göttingen, Germany;Universidade Federal do Pará, Belém, Brazil;

关键词: antibiotic resistance;    genomic sequencing;    mobile genetic elements;    urinary tract infection;    UPEC;   

DOI  :  10.3389/fmicb.2020.549254

来源: DOAJ

【 摘 要 】

The rapid emergence of multidrug-resistant (MDR) bacteria is a global health problem. Mobile genetic elements like conjugative plasmids, transposons, and integrons are the major players in spreading resistance genes in uropathogenic Escherichia coli (UPEC) pathotype. The E. coli BH100 strain was isolated from the urinary tract of a Brazilian woman in 1974. This strain presents two plasmids carrying MDR cassettes, pBH100, and pAp, with conjugative and mobilization properties, respectively. However, its transposable elements have not been characterized. In this study, we attempted to unravel the factors involved in the mobilization of virulence and drug-resistance genes by assessing genomic rearrangements in four BH100 sub-strains (BH100 MG2014, BH100 MG2017, BH100L MG2017, and BH100N MG2017). Therefore, the complete genomes of the BH100 sub-strains were achieved through Next Generation Sequencing and submitted to comparative genomic analyses. Our data shows recombination events between the two plasmids in the sub-strain BH100 MG2017 and between pBH100 and the chromosome in BH100L MG2017. In both cases, IS3 and IS21 elements were detected upstream of Tn21 family transposons associated with MDR genes at the recombined region. These results integrated with Genomic island analysis suggest pBH100 might be involved in the spreading of drug resistance through the formation of resistance islands. Regarding pathogenicity, our results reveal that BH100 strain is closely related to UPEC strains and contains many IS3 and IS21-transposase-enriched genomic islands associated with virulence. This study concludes that those IS elements are vital for the evolution and adaptation of BH100 strain.

【 授权许可】

Unknown