Deep Eutectic Solvents as Smart Cosubstrate in Alcohol Dehydrogenase-Catalyzed Reductions

Catalysts

SantiagoNahuel Chanquia¹  Lei Huang¹  Selin Kara¹  Guadalupe García Liñares²  Pablo Domínguez de María³ 

[1] Department of Engineering, Biocatalysis and Bioprocessing Group, Aarhus University, 8000 Aarhus, Denmark;Organic Chemistry Department, UMYMFOR-CONICET, Laboratory of Biocatalysis, Faculty of Exact and Natural Sciences, University of Buenos Aires, Buenos Aires C1428EGA, Argentina;Sustainable Momentum, SL. Av. Ansite 3, 4-6, Las Palmas de Gran Canaria, 35011 Canary Islands, Spain;

关键词: deep eutectic solvents;    alcohol dehydrogenases;    smart cosubstrate;    reductions;    cinnamyl alcohol;   

DOI  :  10.3390/catal10091013

来源: DOAJ

【 摘 要 】

Alcohol dehydrogenase (ADH) catalyzed reductions in deep eutectic solvents (DESs) may become efficient and sustainable alternatives to afford alcohols. This paper successfully explores the ADH-catalyzed reduction of ketones and aldehydes in a DES composed of choline chloride and 1,4-butanediol, in combination with buffer (Tris-HCl, 20% v/v). 1,4-butanediol (a DES component), acts as a smart cosubstrate for the enzymatic cofactor regeneration, shifting the thermodynamic equilibrium to the product side. By means of the novel DES media, cyclohexanone reduction was optimized to yield maximum productivity with low enzyme amounts (in the range of 10 g L⁻¹ d⁻¹). Notably, with the herein developed reaction media, cinnamaldehyde was reduced to cinnamyl alcohol, an important compound for the fragrance industry, with promising high productivities of ~75 g L⁻¹ d⁻¹.

【 授权许可】

Unknown