Alterations in intestinal microbiota by alginate oligosaccharide improve intestinal barrier integrity in weaned pigs

Journal of Functional Foods

Jun He¹  Xiangbing Mao²  Jin Wan²  Daiwen Chen²  Zhiqing Huang²  Ping Zheng²  Jie Yu²  Jiao Zhang²  Bing Yu² 

[1] Corresponding author.;Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, Sichuan, People’s Republic of China;

关键词: Alginate oligosaccharide;    Intestinal microbiota;    Microbial metabolites;    Inflammatory reactions;    Intestinal barrier integrity;    Weaned pigs;   

DOI  :  

来源: DOAJ

【 摘 要 】

In this study, we investigated the effects of alginate oligosaccharide (AOS) on the intestinal microbiota and intestinal barrier integrity in weaned pigs. Twenty-four weaned pigs were equally assigned to either a control group (fed the basal diet) or an AOS group (fed the basal diet supplemented with 100 mg/kg AOS). AOS upregulated caecal and colonic tight junction protein expression levels but downregulated caecal pro-inflammatory cytokine expression levels. AOS increased the caecal and colonic abundance of phospho-adenosine 5′-monophosphate-activated protein kinase α (p-AMPKα) and decreased the abundance of caecal intra-nuclear nuclear factor-κB (NF-κB) p65. Furthermore, AOS increased the caecal prevalence of several bacteria belonging to Bacteroidetes and Firmicutes as well as the caecal and colonic concentrations of short-chain fatty acids (SCFAs). Thus, AOS alters the intestinal microbiota composition and SCFA concentrations in weaned pigs, thereby influencing the intestinal inflammatory responses and barrier integrity by regulating NF-κB and AMPK pathways.

【 授权许可】

Unknown